提取分离工艺对白头翁皂苷的影响  

Extractive and Separation Technology of Pulchinenoside

作　　者：宋科标[1] 时维静[1] 周杰[1] 王贵阳[1] 韩伟[1] 

机构地区：[1]安徽科技学院食品药品学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2011年第5期42-46,共5页Journal of Anhui Science and Technology University

基　　金：安徽科技学院大学生创新课题;安徽科技学院自然科学研究项目(ZRC2011294);安徽省中药材产业技术研发中心基金资助

摘　　要：优选最佳白头翁皂苷B4提取与分离工艺。本试验采用不同萃取工艺分离白头翁皂苷;采用高效薄层色谱法、高效液相色谱法,通过比较得率、白头翁皂苷B4含量等,考察提取分离工艺对白头翁皂苷的影响。结果:工艺Ⅱ优于工艺Ⅰ。工艺Ⅱ不仅提取得率高,且皂苷B4集中在正丁醇部分。本项研究对白头翁皂苷提取分离工艺的选用提供了试验依据。To study extraction and separation technology of pulchinenoside, pulsatillae was extracted by different abstraction technologyies. Pulchinenoside B4 content and yield were detected by HPLC and HPTLC. The results showed that technology Ⅱ is better than technology 1. Pulchinenoside B4 with high yield can be obtained by this technology Ⅱ and Pulchinenoside B4 was concentrated in n -butanol part. This research provides a test basis for B4 separation and purification.

关 键 词：白头翁皂苷B4 极性 高效液相 薄层色谱 

分 类 号：S853.73[农业科学—临床兽医学]