黄鳍鲷(Sparus latus)两种生长激素受体的cDNA克隆及组织表达分析  

cDNAs CLONING AND TISSUES EXPRESSION OF TWO GROWTH HORMONE RECEPTORS IN YELLOW-FIN BREAM SPARUS LATUS

作　　者：马细兰[1,2,3] 冷婷婷[1] 刘启智[1] 周立斌[3] 张勇[1] 林浩然[1] 

机构地区：[1]中山大学有害生物控制与资源利用国家重点实验室生命科学学院水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室,广州510275 [2]华南师范大学生命科学学院,广州510631 [3]惠州学院生命科学系生物技术研究所,惠州516007

出　　处：《海洋与湖沼》2011年第6期830-838,共9页Oceanologia Et Limnologia Sinica

基　　金：国家重点基础研究发展计划(973计划)项目;2010CB126302号;广东省自然科学基金资助项目;9451601501001986号;广东省教育部产学研结合项目;2010B090400551号

摘　　要：采用RT-PCR方法克隆了黄鳍鲷两种生长激素受体(Growth hormone receptor,GHR)的cDNA序列,序列分析表明:GHR1开放阅读框为1935bp,共编码645个氨基酸,GHR2开放阅读框为1749bp,共编码583个氨基酸,GHR1与GHR2的氨基酸同源性为36.7%。GHR1和GHR2在分子结构上存在显著差异,GHR1胞外域有7个半胱氨酸残基而GHR2只有6个;GHR1胞内域有9个酪氨酸残基而GHR2只有5个,两者的结构差异表明两者可能具有不同的生物学功能。用Real-timeRT-PCR方法研究了GHR1和GHR2在各组织的分布情况,结果表明:GHR1和GHR2在所检测的10种组织中均有表达,其中以肝脏、肌肉、垂体表达量较高,且在大部分组织中GHR2表达量均高于GHR1。Growth hormone （GH） has many important physiological roles in the control of growth, metabolism and reproduction, which is mediated by growth hormone receptor （GHR）. In this study, two cDNAs encoding GHR were isolated from the liver of yellow-fin bream Sparus latus. The two cDNAs, one consisting of 1935bp and the other of 1749bp, encoding for putative 645- and 583-amino acid GHR （designated GHR1 and GHR2, respectively）, shared 36.7% identity in deduced amino acid sequence. GHR1 and GHR2 showed the conserved structural characteristics of GHR family, including the FGEFS motif, the boxl and box2 regions, extracellular cysteine residues and intracellular tyrosine residues. However, there were differences of structural features between the two receptors as well. GHR2 lacked one pair of extracellular cysteines and 4 intracellular tyrosine residues which were conservative in GHR1. The structural discrepancies thus undoubtedly indicated the distinct biological functions of GHR1 and GHR2. Real-time RT-PCR analysis showed that both GHR1 and GHR2 mRNAs were presented in all tissues tested and expressed extremely highly in the liver. In most tissues, GHR2 expressed significantly higher than GHR1. The expression distinction of GHR1 and GHR2 in pituitary and peripheral tis- sues like liver, muscle, spleen, kidney and gonad indicated that the two receptors may have different biological functions.

关 键 词：黄鳍鲷 生长激素受体 CDNA克隆 REAL-TIME RT-PCR 组织表达 

分 类 号：Q95[生物学—动物学]