AGPase基因转化木薯获得转基因植株的研究  

Development of Transgenic Cassava Plants Using AGPase Gene Transformation

作　　者：郭运玲[1] 姚庆荣[1] 孔华[1] 刘恩平[1] 贺立卡[1] 郭安平[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带作物学报》2011年第12期2274-2277,共4页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.30960204);现代农业产业技术体系项目(No.nycytx-17);国家重点基础研究发展计划(973)项目(No.2010CB126605)

摘　　要：通过农杆菌介导法将一个提高淀粉含量的AGPase基因在块根特异启动子的调控下成功导入木薯华南8号(SC8)基因组,并获得转化抗性再生植株25株,转化率为1.20%。经生根初筛有19株转化植株能在抗性生根培养基生根,经PCR、Southern等分子检测证实这19株转化植株为转基因植株。A GPase gene functioning to increase starch content under the control of root tuberous specific expression promotor（Sporamin）was successful introduction into cassava SC8 genome by Agrobacterium-mediated transformation. 25 transgenic cassava plants were obtained, and the transformation frequency was 1.20%. 19 of 25 transformed plants can root in rooting resistance medium： PCR and Southern blot analysis showed that AGPase gene was integrated into the genome of 19 transgenic cassava plants.

关 键 词：AGPase基因 木薯 转基因植株 农杆菌介导 

分 类 号：S533[农业科学—作物学] Q78[生物学—分子生物学]