中国水仙查尔酮异构酶基因的克隆与表达分析  

Cloning and Expression Analysis of Chalcone Isomerase Gene from Narcissus tazetta var.chinensis

作　　者：蔡雪玲[1,2] 陈晓静[1,2] 叶一江[1,2] 何玮毅[3] 申艳红[1,2] 

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学园艺植物遗传育种研究所,福建福州350002 [3]福建农林大学应用生态研究所,福建福州350002

出　　处：《热带作物学报》2011年第12期2287-2292,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.30972031);福建省科技重大专项专题(No.2010NZ0003-2)

摘　　要：查尔酮异构酶(CHI)是影响类黄酮合成的一个重要限速酶,在植物花色发育过程中起着重要作用。通过RT-PCR和RACE技术从中国水仙的花瓣中克隆得到3条CHI基因,分别命名为NtCHIW、NtCHIJ和NtCHIY。3个基因均含有一个735 bp的开放阅读框(ORF),编码244个氨基酸。氨基酸序列分析表明:白花水仙与金盏银台、黄花水仙、洋葱、粳稻、拟南芥、油棕榈、葡萄相应基因的氨基酸序列同源性分别为93.03%、93.44%、64.75%、55.42%、50.58%、59.43%、58.20%。荧光定量PCR分析表明:随着花开放的过程,中国水仙的CHI基因转录水平发生变化,说明CHI基因可能参与花色变化的过程。Chalcone isomerase（CHl）is one of the key enzymes in the biosynthesis pathway of flavonoids and plays an important role in the process of flower color development. Three genes, named NtCHIW, NtCHIJ and NtCHIY respectively, were cloned from Narcissus tazetta var. chinensis petal by RT-PCR and RACE. The open reading frame encompassed 735 hp encoding a polypeptide of 244 amino acids. Sequencing analysis showed that the amino acid sequence of Baihna Narcissus was 93.03%, 93.44%, 64.75%, 55.42%, 50.58%, 59.43%, and 58.20% homologous with CHI genes from Jinzhanyintai Narcissus, Huanghua Narcissus, AUium cepa, Elaeis oleifera, Vitis labrusca, Oryza sativa Japonica, Arahidopsis thaliana, respectively. The result of real time RT-PCR showed that the transcription expression of CHI gene changed accordingly during flower blooming, indicating the possible role of CHI genes in the change of flower color.

关 键 词：中国水仙 查尔酮异构酶 基因克隆 序列分析 表达分析 

分 类 号：S682.21[农业科学—观赏园艺]