水稻蛋白磷酸酶ABI2基因启动子的克隆和功能分析  

Cloning and Functional Analysis of Rice Protein Phosphatase ABI2 Gene Promotor

作　　者：牟少亮[1,2] 李秀娟[1,2] 官德义[3,2] 赖燕[1,2] 何水林[3,2] 

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]作物遗传改良与综合利用教育部重点实验室,福建福州350002 [3]福建农林大学作物科学学院,福建福州350002

出　　处：《热带作物学报》2011年第12期2293-2297,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金资助项目(No.30971718);国家转基因生物新品种培育重大专项(No.2009ZX08001-015B)资助

摘　　要：分离了水稻PP2C家族中的一个成员OsABI2的启动子,测序鉴定后构建其GUS融合表达载体,通过农杆菌介导的方法获得水稻转基因植株。GUS蛋白组织化学染色结果表明,OsABI2启动子驱动的GUS基因在水稻根茎叶中都有表达,在根中表达量最高,叶中表达量最低,而且根部分生组织区明显高于根部其他组织。另外在萌发的芽中也有明显表达。OsABI2启动子在T1代转基因植株的诱导表达的GUS蛋白活性分析结果表明,在机械损伤、稻瘟病、干旱逆境和ABA、MeJA和SA处理条件下,OsABI2启动子驱动的GUS活性都出现了明显上调。上述研究结果说明,OsABI2很可能参与了发育,生物和非生物逆境胁迫的调控。The promotor of one PP2C gene named OsABI2 was cloned, sequenced and fused with GUS gene. The transgenie rice was obtained by an Agrobacterium-mediated method. The results of histochemical GUS staining showed the activity of GUS driven by OsABI2 promotor was the highest in roots especially in meristem, and the lowest in leaves. There was significant expression in germination buds as well. The GUS activity induced by the promotor of OsABI2 in T1 generation was determined by quantitative assay. The results showed that GUS activity was increased significantly by stresses（wounding, rice blast and drought）and hormones treatmnt （ABA, MeJA and SA）. It is concluded that OsABI2 played a role in the regulation of development, biotic and abiotie stresses.

关 键 词：OsABI2 启动子 功能分析 

分 类 号：Q74[生物学—分子生物学]