香蕉枯萎病菌ste12基因的克隆与序列分析  

Cloning and Sequence Analysis of ste12 Gene from Fusarium oxysporum f.sp.cubense

作　　者：周端咏[1,2] 刘一贤[1,2] 谢德啸[1,2] 黄小娟[1,2] 魏巍[1,2] 郭立佳[2] 杨腊英[2] 黄俊生[2] 

机构地区：[1]海南大学,海南海口570100 [2]中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州571737

出　　处：《热带作物学报》2011年第12期2298-2301,共4页Chinese Journal of Tropical Crops

基　　金：国家公益性行业(农业)科研专项"由尖孢镰刀菌引起的作物土传病害综合防控技术研究"(No.200903049);中央级公益性科研院所基本科研业务费专项(No.2011HZS1J022)

摘　　要：为了解ste12基因在尖孢镰刀菌古巴专化型侵染香蕉过程中的作用,及其与尖孢镰刀菌古巴专化型1号和4号生理小种之间的致病力差异关系,采用PCR和RT-PCR方法扩增了2个生理小种的ste12基因,并对扩增产物进行了测序及相似序列搜索和比对,还对基因编码的蛋白进行了氨基酸序列比对和分析。研究结果表明,2个生理小种ste12基因开放阅读框均为2070 bp,存在7个碱基的差异,基因同源性为99.7%。编码689个氨基酸,氨基酸序列一个有差异。根据生物信息学软件预测编码蛋白没有信号肽,具有两个相同的功能位点,分子量和PI分别为75 ku和6.47。Fusarium oxysporum f.sp.cubense（FOC）is the cause of the Panama disease.ln order to know the roles of stel2 gene in invasion process of FOC into banana plants, and to determine the difference in pathogenicity of the race l and 4 of FOC to the same banana cuhivar, the stel2 genes were amplified by polymerase chain reaction and reverse transcription polymerase chain reaction, and the amplification products were then sequenced. After that, the nucleotide sequences of the stel2 gene and the sequences of their products were aligned using DNAStar Meglignment software. The results revealed that the length of the open reading frames of stel2 genes from both races of Fusarium oxysporum f.sp.cubense were 2 070 bp, and they encoded a polypeptide of 689 amino acids with 75 ku of calculated molecular weight and 6.47 of pI.The stel2 gene from FOC race 1 showed 99.7% similarity to that of FOC race 4. Besides that,there were seven differences in nucleotide sequence and only one differencein amino acid sequence.

关 键 词：尖镰刀菌古巴专化型 生理小种 ste12基因 致病性 转录因子 

分 类 号：S437.67[农业科学—农业昆虫与害虫防治]