基于DNA序列的12种粉蚧亲缘关系分析  

Genetic Relationship of 12 Species of Mealybugs(Hemiptera: Pseudococcidae)Based on DNA Sequences

作　　者：何衍彪[1,2] 万宣伍[2] 詹儒林[1] 孙光明[1] 刘映红[2] 许再福[3] 赵艳龙[1] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所,广东湛江524091 [2]西南大学植物保护学院,重庆400716 [3]华南农业大学资源环境学院,广东广州510640

出　　处：《热带作物学报》2011年第12期2324-2330,共7页Chinese Journal of Tropical Crops

基　　金：公益性行业科技专项-菠萝现代产业技术体系建立与研究(No.3-41);中国热带农业科学院中央级非盈利性研究机构基本科研业务费研究专项(No.sscri200702)

摘　　要：对菠萝洁粉蚧、新菠萝灰粉蚧、大洋臀纹粉蚧、柑橘臀纹粉蚧、康氏粉蚧、榕树粉蚧、热带蔗粉蚧、扶桑绵粉蚧、湿地松粉蚧等9种粉蚧(14个种群)rDNA 18S、28S进行测序,结合GenBank中同源序列,通过对12种粉蚧的18S、28S序列进行多重排比和聚类,探讨rDNA 18S、28S用于粉蚧系统进化分析及粉蚧分子鉴定的可行性。序列分析结果表明:rDNA18S、28S序列比较保守,适合于菠萝洁粉蚧、新菠萝灰粉蚧、康氏粉蚧、热带蔗粉蚧、扶桑绵粉蚧、湿地松粉蚧、长尾粉蚧等粉蚧种间的分子鉴定;但柑橘臀纹粉蚧、大洋臀纹粉蚧、无花果臀纹粉蚧的rDNA 18S、28S序列具有高度相似性,18S、28S不适合用于这3个近缘种的分子鉴定,其mtDNA COI序列变异率相对较高,可作为其分子鉴定的依据;而榕树粉蚧2个种群rDNA 18S、28S序列遗传距离约0.014,该蚧虫可能是至少含有2个隐存谱系或姊妹种的复合种。该结果为我国粉蚧的遗传进化和分子鉴定提供了参考。The rDNA 18S and 28S of 14 populations of 9 species of mealybug, including Dysmicoccus brevipes, D. neobrevipes, Planococcus minor, Pseudococcus citri, P. Comstock, P. baliteus, Saccharicoccus sacchari, Phenacoccus solenopsis, Oracella acuta were sequenced. These sequences were analyzed with ClustalX 2.0, MEGA 4.1 and PAUP 4.10 and so on, and the homological sequences of other 3 species available in GenBank retrieved for DNA alignment and clustering. The feasibility of the application of 18S ＆ 28S sequences to the phylogenetic reconstruction and molecular identification of the 12 species of mealybug was then evaluated. Alignment of sequenced 18S ＆ 28S showed that they were highly conserved within intraspecific taxa but that significant genetic distances existed between interspecific taxa. Comparison and analysis of the genetic distance led to the clear conclusion that 18S ＆ 28S are suitable only for species-level identification. Sequences of rDNA 18S ＆28S failed to distinguish P. minor, P. cirri, and P. ficus from each other for their highly similarity, and the difference of mtDNA COI sequences of them are enough to distinguish each other. The genetic distance between the collections of P. baliteus from two different hosts was approximately 0.014. These data suggest that P. baliteus might be a species complex which includes at least two cryptic lineages or sibling species.

关 键 词：粉蚧科 核糖体18S/28S基因 线粒体COI基因 亲缘关系 

分 类 号：S436.611.2[农业科学—农业昆虫与害虫防治]