等电聚焦强度对木薯叶片蛋白质双向电泳分离结果的影响  

Effect of Isoelectrofocusing Intensity on Protein Separation of Cassava Leaves by Two-Dimensional Gel Electrophoresis

作　　者：王海燕[1,2,3] 王斌[1,2,3] 王丹[2] 王东阳[1,2,3] 王力敏[1,2] 王红岩[2] 王旭初[2] 田维敏 

机构地区：[1]海南大学农学院,海南儋州571737 [2]中国热带农业科学院热带生物技术研究所,海南海口571101 [3]省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南儋州571737

出　　处：《热带作物学报》2011年第12期2367-2372,共6页Chinese Journal of Tropical Crops

基　　金：国家重点基础研究发展计划(973)项目(No.2010CB12660-2);中国热带农业科学院生物所基本科研业务费(No.ITBB110101)

摘　　要：通过优化等电聚焦强度改良木薯叶片蛋白质双向电泳的分离效果。结果表明,13万Vhs等电聚焦强度能很好地分离木薯叶片蛋白质,且分离的蛋白质具有良好的质谱兼容性。质谱鉴定7个蛋白点,结果显示这些蛋白主要参与能量代谢,其中有些蛋白点是在13万Vhs聚焦强度下特异出现的。To obtain high-resolution reference of two-dimensional gel electrophoresis （2-DE）profiles of proteins from cassava leaves, the parameters of isoelectro-focusing（IEF）intensity were optimized. Results showed that the quality of 2-DE profiles under 130 000 Vhs was better than that of those under other conditions. Seven proteins among the selected 10 proteins were positively identified by MALDI TOF MS, suggesting their MS compatibility. The identified proteins were mainly involved in energy metabolic pathway and some of which appeared only in the 2-DE profiles under 130000 Vhs condition.

关 键 词：木薯 叶片蛋白 等电聚焦 双向电泳 

分 类 号：S889.5[农业科学—特种经济动物饲养]