基于GC-MS联用技术的毛竹挥发性次生代谢产物分析  

GC-MS analysis of volatile secondary metabolites in Phyllostachys heterocycla 'Pubescens'

作　　者：刘志明[1] 任海青[2] 蒋乃翔[1] 

机构地区：[1]东北林业大学材料科学与工程学院生物质材料科学与技术教育部重点实验室,黑龙江哈尔滨150040 [2]中国林业科学研究院木材工业研究所,北京100091

出　　处：《经济林研究》2011年第4期81-84,共4页Non-wood Forest Research

基　　金："十一五"国家科技支撑计划项目中的子课题(2006BAD19B04)

摘　　要：为给毛竹代谢产物的分析提供基础数据,采用气相色谱-质谱联用技术,对毛竹挥发性次生代谢产物中的活性化合物进行了分析。结果表明:3、5、7年生不同竹龄毛竹竹秆顶部和5年生毛竹竹秆中部和底部的挥发性次生代谢产物均以碳氢化合物为主,其共有的化合物为碳原子个数在22～33之间的烷烃;1年生毛竹竹秆的顶部、5年生毛竹竹秆的中部和底部、7年生毛竹竹秆的底部都含有柏木醇;7年生毛竹竹秆底部挥发性次生代谢产物的提取得率最高(0.045%),且含有质量分数较高的柏木醇。柏木醇是具有开发潜力的活性化合物。In order to provide basic data for metabolite analysis,the active compounds of volatile secondary metabolites in Phyllostachys heterocycla ＇Pubescens＇ were analyzed with gas chromatography-mass spectrometry（GC-MS）technology.The results showed that main volatile secondary metabolites were hydrocarbons in the top culm of 3,5 and 7 years old bamboo and in the middle and bottom culm of 5 years old bamboo,and the mutual compounds were the alkanes with 22-33 carbon atoms.Cedrol was in the top culm of one-year-old bamboo,in the middle and bottom culm of five-year-old bamboo,and in the bottom culm of seven-year-old bamboo.Extraction yield of volatile secondary metabolites was the highest（0.045%）,and the mass fraction of cedrol was higher in the bottom culm of seven-year-old bamboo.Cedrol was a kind of active compound with development potential.

关 键 词：毛竹 次生代谢产物 柏木醇 气相色谱-质谱分析 

分 类 号：S795.7[农业科学—林木遗传育种]