痛泻要方对腹泻型肠易激综合征模型大鼠结肠组织血管活性肠肽及受体1表达影响  

Effects of Tongxieyaofang on Vasoactive Intestinal Polypeptide and VPAC1 in Rats of Diarrhea-predominant Irritable Bowel Syndrome

作　　者：许惠娟[1] 滕超[1] 钱永清[2] 刘慧慧[1] 王艳杰[1] 柴纪严[1] 王德山[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]吉林大学白求恩医学院,吉林长春130021

出　　处：《中华中医药学刊》2012年第2期268-270,I0004,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(2007CB512702)

摘　　要：目的:探讨痛泻要方治疗腹泻型肠易激综合征(D-IBS)模型大鼠的作用机制。方法:采用应激与束缚相结合的方法复制D-IBS模型后,应用免疫组化、逆转录聚合酶链反应(RT-PCR)检测结肠组织血管活性肠肽(VIP)与其受体1(VPAC1)蛋白和mRNA的表达。结果:与正常组比较,模型组结肠组织VIP与VPAC1蛋白、mRNA表达均上调(P<0.01);与模型组比较,痛泻要方组结肠组织VIP与VPAC1蛋白、mRNA的表达则下调(P<0.01)。结论:痛泻要方治疗D-IBS的作用机制与结肠组织VIP与VPAC1表达下调有关。Objective ：To study the mechanism of Tongxieyaofang （TXYF） on rat model of diarrhea -predominant irritable bowel syndrome （D -IBS）. Methods ：Model rats of D -IBS were made by stress and bound method. The method of immunohistochemistry was used to determine the expressions of VIP and VPAC1 in colon tissue. In addition, reverse transcription - polymerase chain reaction （ RT - PCR） was applied to analyze the expressions of VIP and VPAC1 mRNA in colon tissue. Results ： The expressions of VIP and VPAC1 mRNA and protein in colon of TXYF group were lower than those of the model group, but higher than those of the control group. Conclusion ： The effect of TXYF on model rats of D -IBS is related to VIP and VPAC1 expression levels.

关 键 词：痛泻要方 腹泻型肠易激综合征 血管活性肠肽 血管活性肠肽受体1 

分 类 号：R285.5[医药卫生—中药学]