大黄酸对内毒素血症小鼠保护作用的实验研究  

Experimental Study on Protective Effects of Rhein Treating Endotoxemia in Rats

作　　者：马超英[1] 彭春荣[1] 耿耘[1] 杨超[1] 涂显琴[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《中华中医药学刊》2012年第2期288-289,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30873365);中央高校基本科研业务费专项研究资助项目(SWJTU09ZT29)

摘　　要：目的:探讨大黄酸对内毒素血症小鼠的保护作用。方法:将45只雄性昆明小鼠随机分为空白组、模型组和大黄酸组,每组15只。空白组腹腔消毒注射生理盐水(10mg/kg),其余各组腹腔消毒注射LPS(10mg/kg)。治疗组于攻毒前0.5h、攻毒后1h和取血前0.5h给予大黄酸1.4mg.10g-1.d-1灌胃,空白组和模型同时按比例给予等量生理盐水。8 h后在无菌条件下采集心室血0.5mL进行血浆ET检测,取部分肝脏进行免疫组化和生化检测,取肝进行组织学观察。结果:大黄酸组对小鼠内毒素、TNF-α、IL-1β、IL-10、MDA、SOD和肝组织病理炎症等改善优于其他组。结论:大黄酸对小鼠内毒素血症有显著的保护作用,其途径可能通过改善微循环,改善肝脏的能量代谢,减轻肝脏的脂质过氧化反应,降低机体的内毒素水平,从而减少炎性因子的产生,阻断内毒素血症的生物学效应。Objective： To explore the protective effect of Rhein on end0toxemia. Methods： Divide 45male KM mice into 3 groups randomly： control group; model group and rhein group, 15 KM male mice each group. Mice of control group are abdominal injected NS（ 10mg / kg） sterile,the others are abdominal injected LPS（ 10mg / kg）. The treatment groups are rhein liquid （1.4mg/10g · d） by Ig at 0.5h before and lh after attacking and 0.5h before collecting blood; the control group and model group are given NS at the same time. 8 hours later, collect 0.5mL ventricular blood under sterile conditions and detect the plasma ET, collect parts of the liver to have the immunohistochemistry and biochemical measurement, and take the liver to have the histological analysis. Results ： The effect of rhein group and ethyl group are superior to other groups on improving the endotoxin,TNF-α、IL-1β、IL-10 ,MDA,SOD and the pathological inflammatory activity of liver. Conclusions ： Rhein has obvious effect on Endotoxemia, the mechanisms are the followings ： improving microcirculation;improving the metabolism of liver;reducing lipid peroxidation of liver; decreasing the level of endotoxin; and thus reducing the release of proinflammatory cytokines and blocking the biological effects of endotoxin play.

关 键 词：大黄酸 内毒素血症 实验研究 

分 类 号：R285.5[医药卫生—中药学]