复方地黄对帕金森病大鼠多巴胺能神经元及GDNF表达的影响  

Effects of Compound Rehmannia on Dopaminergic Neurons And the Expression of GDNF in Parkinsonian Rats

作　　者：胡乔[1] 王炼[1] 刘云[1] 包琼琼[1] 庄晓赛[1] 周莉莉[1] 胡艳兵[1] 孙妙璇[1] 张雄[1] 

机构地区：[1]温州医学院附属第二医院神经科,浙江温州325027

出　　处：《中华中医药学刊》2012年第2期321-323,I0011,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(Y207557);浙江省中医药局资助项目(2010ZB103);温州市科委资助项目(H20100074)

摘　　要：目的:研究复方地黄对6-羟基多巴胺(6-hydroxydopamine,6-OHDA)诱导的帕金森病(Parkinson’s disease,PD)大鼠多巴胺能神经元的保护作用及其可能机制。方法:40只SD雄性大鼠,分为正常组、假手术组、模型组、模型组+复方地黄组(即干预组)。观察PD大鼠药物诱发的旋转行为;免疫组化法观察黑质酪氨酸羟化酶(tyrosine hydroxylase,TH)阳性神经元数目及胶质细胞源性神经营养因子(glial cell line-derivedneurotrophic factor,GDNF)表达水平的变化。结果:干预组与模型组间的旋转圈数有明显差异(P<0.05);模型组黑质TH阳性神经元数目(52.9±2.2)明显少于正常组(160.3±2.4)、假手术组(161.6±3.4)及干预组(61.5±2.6)(P<0.05);在大鼠黑质和纹状体部位,干预组GDNF阳性细胞数量比正常组、假手术组、模型组均明显增加(P<0.05)。结论:复方地黄方能明显改善PD大鼠的旋转行为,拮抗6-羟基多巴胺诱导的PD大鼠黑质多巴胺能神经元的丢失,其保护作用可能与促进内源性GDNF分泌增加有关。Objective： To investigate the neuroprotective effects of compound rehmannia on dopaminergic neurons of Parkinsonian rat model induced by 6 -hydroxydopamine（6 -OHDA）. Methods： The SD rats were divided into 4 groups： normal control group, sham operated group,6 -OHDA group, compound rehmannia ＋ 6 -OHDA group . The rotation behaviour in PD rats were observed. The number of tyrosine hydroxylase （TH） positive cells in the substantia nigra and the expression of glial cell line -derived neurotrophic factor （GDNF） were measured by immunohistochemistry. Results： The rats in compound rehmannia ＋ 6 - OHDA group appeared obvious changes of rotation behaviour（ P 〈 0.05）. The number of TH positive cells in the substantia nigra was significantly reduced in 6 - OHDA group than that in compound rehmannia ＋ 6 -OHDA group（P 〈 0.05 ）. The expression of GDNF in the substantia nigra and striatum was markedly increased in compound rehmannia ＋ 6 - OHDA group than that in the other groups （ P 〈 0. 05 ）. Conclusion ： Compound rehmannia could significantly improve the rotation behaviour of PD model rats, and partially block the loss of dopaminergic neurons of Parkinsonian rat model induced by 6 - OHDA. The mechanism of neuroprotective effect may be related to the elevated secretion of endogenous GDNF.

关 键 词：复方地黄 帕金森病 酪氨酸羟化酶 胶质细胞源性神经营养因子 

分 类 号：R285.5[医药卫生—中药学]