HPLC测定活血止痛膏中芍药苷、马钱子碱、士的宁的含量  

HPLC Determination of Peoniflorin,Brucine,Strychnine in Huoxue Zhitong Plaster

作　　者：刘晓昱[1] 王佩[1] 成羿[1] 

机构地区：[1]杭州市中医院中药实验室,浙江杭州310007

出　　处：《中华中医药学刊》2012年第2期330-332,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省科技计划资助项目(2009C33100)

摘　　要：目的:建立高效液相色谱法测定活血止痛膏中芍药苷、马钱子碱、士的宁的含量。方法:采用AgilentTC-C18色谱柱(250 mm×4.6 mm,5μm),柱温为25℃,流速为1 mL.min-1;芍药苷的流动相为乙腈-水(16∶84),检测波长为230 nm;马钱子碱、士的宁的流动相为乙腈-0.01mol.L-1庚烷磺酸钠与0.02mol.L-1磷酸二氢钾等量混合溶液(用10%磷酸溶液调节pH值至2.8)(21∶79),检测波长为260 nm。结果:两种液相方法可分别测定活血止痛膏中芍药苷、马钱子碱、士的宁的含量。芍药苷浓度在25.2～200.2μg.mL-1(r=0.9996),马钱子碱浓度在2.45～19.6μg.mL-1(r=0.9998),士的宁浓度在2.95～23.6μg.mL-1(r=0.9997)范围内与其峰面积呈良好的线性关系;平均回收率(n=9)分别为95.13%(RSD=1.77%),87.63%(RSD=2.99%),90.93%(RSD=2.57%)。结论:两种液相测定方法专属性强,结果准确,可用于活血止痛膏的质量控制。Objective： To establish an HPLC method to determine the contents of peoniflorin, brucine and strychnine in Huoxue Zhitong Plaster. Mthod： Agiltnt C18 was employed. The column temperature was 25 ℃ and the flow rate was 1 mL · min^-1. Acetonitrile-water（ 16：84） was adopted as the mobile phase of peoniflorin and the UV detection wavelength was 230nm. A mixture of acetonitrile and a mixture solution of equal quantities of 0.01mol · L-1 sodium heptane- sulfonate and 0.02mol · L-1potassium dihydrogen phosphate （adjust pH to 2.8 with 10% phosphoric acid） （20 ： 80） were adopted as the mobile phase of brucine, strychnine and the UV detection wavelength was 260nm. Result ： The contents of three components could be determined respectively by two HPLC methods. The calibration curves were linear in the ranges of 25.2 - 200.21μg.mL-1 （ r = 0. 9996 ） for peoniflorin, 2.45 - 19.6 μg.mL-1 （ r = 0. 9998 ） for brucine, 2.95 - 23.6μg.mL-1 （r = 0. 9997） for strychnine. The average recoveries （ n = 9） of peoniflorin, brucine and strychnine were 95.13% （ RSD = 1.77% ） ,87.63% （ RSD = 2.99% ） ,90.93% （ RSD = 2.57% ） respectively. Conclusion ： The two methods were accurate with good reproducibility and strong specificity. They were suitable for determination of three compounds in Huoxue Zhitong Plaster.

关 键 词：高效液相色谱法 活血止痛膏 芍药苷 马钱子碱 士的宁 

分 类 号：R927.2[医药卫生—药学]