黄芪失笑散对急性心肌梗死大鼠Caspase-3、Bax及Bcl-2表达的影响  

Effects of Huangqi Shixiao Powder on Caspase-3、Bax and Bcl-2 Expressions in Rats with Acute Myocardial Infarction

作　　者：赫小龙[1] 陈铁龙[1] 祝光礼[1] 齐国安[1] 

机构地区：[1]浙江中医药大学,浙江杭州310007

出　　处：《中华中医药学刊》2012年第2期357-359,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药管理局资助项目(2008CB054);杭州市科技局资助项目(20080333Q23)

摘　　要：目的:观察不同剂量黄芪失笑散对大鼠急性心肌梗死后细胞凋亡及Caspase-3、Bax及Bcl-2蛋白表达的影响。方法:120只雄性SD大鼠随机分为5组:模型组、对照组、黄芪失笑散高剂量组(28g.kg-1.d-1)、中剂量组(14g.kg-1.d-1)、小剂量组(7g.kg-1.d-1)。连续灌胃7天后,结扎冠状动脉左前降支造成急性心肌梗死模型,对照组不结扎,手术后4.5h留取心脏标本,TTC染色法检测心肌梗死面积,Western blot法检测Caspase-3、Bax及Bcl-2蛋白表达量。结果:与对照组比较,黄芪失笑散组可明显减少心肌梗死面积和Caspase-3、Bax蛋白表达量,升高Bcl-2蛋白表达量,P<0.01。不同剂量组间差异有统计学意义P<0.05,黄芪失笑散大剂量组效果最好。结论:黄芪失笑散能够保护缺血心肌、降低心梗面积,机制与降低梗死边缘区Bax/Bcl-2比值、活化Caspase-3蛋白水平有关。Objective：To investigate the effects of different dosages of Huangqi Shixiao Powder on protein expression levels of Caspase -3 ,Bax and Bc1-2 in rats with acute myocardial infarction. Methods： 120 male SD rats were randomly divided into 5 groups： model group, sham group, large -dose group （28g·kg-1 · d-1, Huangqi Shixiao Powder pretreatment）, medium - dose group （ 14g · kg - 1 ·d - 1, Huangqi Shixiao Powder pretreatment）, and low - dose group （7g · kg- 1 · d - l, Huangqi Shixiao Powder pretreatment） ： After being administered corresponding medicine for 7 days, all groups except sham group underwent coronary ligation of left anterior descending branch to establish acute myocardial infarction models, and sham group only received thoracotomy without coronary ligation. Four and half hours after AMI, myocardium tissues were collected to measure the infarct size by TTC staining and protein expression levels of Caspase - 3, Bax and Bc1-2 by western blot. Results ：The infarct size and Caspase- 3 and Bax expressions decreased significantly in all the treatment groups than that in model group , while Bcl -2 expression increased significantly than those in model group, P 〈 0.01. There are statistic differences among different dosage of Huangqi Shixiao Powder groups, P 〈 O. 05, and the large - dose group receives a better effect than the others in the matter of decreasing infarct size,Caspase - 3 and Bax expressions and increasing Bcl -2 expression. Conclusion： Huangqi Shixiao Powder could decrease infarct size and inhib- it the myocardial apoptosis by decreasing Caspase -3 and regulating the balance of Bax and Bcl -2 expressions.

关 键 词：黄芪失笑散 凋亡 急性心肌梗塞 CASPASE-3 

分 类 号：R285.5[医药卫生—中药学]