五子衍宗方对生精障碍模型大鼠附睾精子参数及血清抑制素B的影响  

Influence of Wuzi Yanzhong Prescription on Epididymo-Semen Sarameters And Serum Inhibitor B of Dyszoospermia Model Rats

作　　者：杨欣[1] 丁彩飞[1] 颜志中[1] 胡慧敏[1] 万凌屹[1] 

机构地区：[1]杭州市红十字会医院,浙江杭州310003

出　　处：《中华中医药学刊》2012年第2期371-373,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划资助项目(2009CA085)

摘　　要：目的:观察五子衍宗方对生精障碍模型大鼠附睾精子参数及血清抑制素B水平的影响。方法:采用随机数字表法将50只雄性SD大鼠分为正常对照组、模型组、甲基睾酮组和五子衍宗方低、高剂量组,每组10只。除正常对照组外,其余各组大鼠按醋酸棉酚50mg/kg体质量灌胃给药,制备生精障碍模型。正常组和模型组大鼠灌服蒸馏水,甲基睾酮组大鼠灌服甲基睾酮混悬液,五子衍宗方低、高剂量组大鼠分别给予低、高浓度(含1.75g、3.5g生药/mL)五子衍宗方混悬液灌胃,每天1次,连续1个月。观察各组大鼠睾丸湿重、睾丸指数及间质细胞数,按WHO标准,采用WLJY-9000荧光染色精子动(静)态图像分析系统检测附睾精子参数,ELISA法检测大鼠血清抑制素B水平。结果:棉酚可造成大鼠睾丸减轻、睾丸指数减小、间质细胞数减少(P<0.01);附睾精子密度降低,活动率和前向运动率(a+b%)下降(P<0.05,P<0.01),血清抑制素B水平降低(P<0.01);五子衍宗方能显著改善大鼠睾丸重量及睾丸指数,增加间质细胞数,改善附睾精子参数(P<0.05,P<0.01),上调血清抑制素B水平(P<0.01),拮抗棉酚造成的大鼠睾丸生精功能损伤。结论:通过上调抑制素B水平,改善睾丸生精功能,可能五子衍宗方治疗不育症的作用机制之一。Objective ： Influence of Wuzi Yanzhong Prescription on epididymo - semen sarameters and serum inhibitor B of dyszoospermia model rats were observed. Methods ： 50 male SD rats were divided into normal control group, model group, methyltestosterone group, low and high dose of Wuzi Yanzhong Prescription groups according to random digits table. Each group had 10 rats. Except the normal control group, other groups were intragastricly administrated with gossypol acetate （50mg/kg）to establish the dyszoospermia model. Distilled water was given to rats in normal and model groups; methyltestosterone was given to rats in methyltestosterone group; Wuzi Yanzhong Decoction with low and high concentration （ 1.75g crude drug/mL;3.5 g crude drug/mL）were given to rats in low and high dose of Wuzi Yanzhong Decoction groups respectively, once a day, lasting for 1 month. Testis wet weight, testis index and interstitial cells number were ＇observed. According to WHO standard, WLJY -9000 was used to measure the epididymo - semen sarameters and ELISA was to measure the level of serum inhibitor B. Results： Gossypol acetate can reduce testis weight, testis index and interstitial cells（ P 〈 0.01 ） ;epididymo -semen density was decreased and activity ration as well as the percentage of progressively moving spermatozoa was decreased （ P 〈 0. 05 ,P 〈 0.01 ）, and the level of serum inhibitor B was decreased（ P 〈 0.01 ）. Wuzi Yanzhong Decoction can improve the wet weight and index of testis, increase the interstitial ceils number and improve the epididymo - semen sarameters （ P 〈 0. 05, P 〈 0.01 ） ;it also can increase the level of serum inhibitor B （ P 〈 0.01 ） ; thus Wuzi Yanzlaong Decoction can relive the spermatogenic function of testis caused by gossypol acetate. Conclusion ： The mechanism of Wuzi Yanzhong Decoction treating sterility may be related to increasing inhibitor B and reliveing the spermatogenic function of testis.

关 键 词：大鼠 附睾 精子 抑制素B 醋酸棉酚 甲基睾酮 五子衍宗方 

分 类 号：R285.5[医药卫生—中药学]