天麻成分D的急性毒性实验及对小鼠睡眠影响的研究  

Research on Acute Toxicity and Mesmerism of Component D of Tall Gastrodia Rhizome by Administered Mouse Intragastrically

作　　者：何晓山[1] 文睿婷[1] 李润梅[1] 山培理[1] 刘红梅[1] 

机构地区：[1]云南中医学院,云南昆明650500

出　　处：《中华中医药学刊》2012年第2期379-381,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：云南省科技计划资助项目(2009ZC092M)

摘　　要：目的:了解天麻成分D灌胃给药的急性毒性,为其进行一步研究提供依据;观察天麻成分D对戊巴比妥钠致小鼠催眠作用的影响。方法:选择SPF级昆明种小鼠为受试对象,采用急性经口毒性试验测定半数致死量(LD50)和最大耐受量(MTD);采用对阈上剂量和阈下剂量戊巴比妥钠所致催眠作用的影响试验来评价药物对中枢神经系统的作用。结果:天麻成分D小鼠ig给药的LD50为1.2069g/kg体重,LD5为0.93464g/kg,MTD为0.8717g/kg;天麻成分D小鼠ig给药,单独使用并不能引起小鼠睡眠,但可以使戊巴比妥钠ip引起的小鼠睡眠作用增强,睡眠潜伏期缩短,睡眠持续时间延长。结论:天麻成分D小鼠ig给药有一定毒性,LD50为1.2069g/kg体重;天麻成分D小鼠ig给药对中枢神经系统可能有一定的抑制作用,不能直接引起小鼠睡眠,但可增强戊巴比妥钠对小鼠的催眠作用。Objective：To get the message of acute toxicity of component D of tall gastrodia rhizome by administered mouse intragastrically and observe its influence on mesmerism induced by pentobarbital sodium. Method：To observe acute toxicity of component D with median lethal dose test determination ; to observe its influence on central nervous system with mice mesmerism induced by suprathreshold dose and subthreshold dose of pentobarbital sodium. Result ： Median lethal dose of component D intragastrically is 1. 6216g/kg, minimum toxic dose is 1. 0482g/kg, and maximally tolerated dose is 0.77 g/kg by weight. Component D cannot cause mice to sleep, but can strengthen mice＇s sleep induced by pentobarbital sodi- um. Conclusion：Component D of tall gastrodia rhizome has a certain toxicity and has some inhibitory action on central nervous system ofmice.

关 键 词：天麻成分D 急性毒性 催眠作用 戊巴比妥钠 

分 类 号：R285.5[医药卫生—中药学]