落叶松脂醇体外抗肿瘤作用的实验研究  

Studies on Anti-tumor Activity in Vitro of Laricircsinol From Patrinia Villosa Juss

作　　者：丁艺雪[1] 李膺[1] 陈旖湛[1] 陈津[1] 张如松[1] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053

出　　处：《中华中医药学刊》2012年第2期420-422,I0017,共4页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:通过体外细胞培养技术考察落叶松脂醇(laricircsinol,以下称LA)对肿瘤细胞的增殖抑制及诱导凋亡作用。方法:采用MTT比色法、生长曲线法考察LA对肿瘤细胞增殖抑制率,荧光显微镜观察LA作用PC-3细胞后的细胞形态,流式细胞术检测LA对PC-3、KB、NB4、B16肿瘤细胞的凋亡率。结果:LA对4种肿瘤细胞均有较强的细胞毒作用,IC50分别为(0.32±0.04)、(3.92±0.09)、(0.59±0.05)、(2.17±0.11)μg.mL-1;生长曲线法结果表明LA对PC-3细胞的抑制作用呈显的著量-效关系,且在一定剂量范围内呈显著的时-效关系;荧光显微镜观察结果表明给药组细胞的细胞核改变和凋亡小体的形成;流式细胞术检测结果表明随LA浓度的增加细胞凋亡率增加,呈显著的量-效关系。结论:LA对多种肿瘤细胞均有抑制作用,诱导细胞凋亡是其作用机制之一。Objective ：To investigate the anti -tumor effect of laricircsinol（ short for LA） from Patrinia viUosa Juss according to in vitro extend experiment on the multiform tumor cells. Methods：The effects of LA on the proliferation were studied on PC -3, KB, NB4 and B16 tumor cells by MTT assay methods, growth curve methods, cell morphological observation and Flow Cytometry（FCM）. Results： The LA had potent cytotoxic effect in vitro on all four tumor cells. The 50% inhibiting concentration （ IC50 ） were 0.32 ±0.04,3.92 ± 0.09,0.59 ±0.05,2.17 ± 0.11μg.mL-1 respectively. The results of growth curve methods indicated that effects of the LA on PC - 3 tumor cells in vitro enhanced as the concentration of LA increased in a certain time. Moreover, it also showed dose - effect and time - effect relationships in a certain range of the LA concentration. The results of cell morphological observation stained by acridine orange/ethidium bromide （AO） indicated that the LA could change the cell core and enhance the fluorescence intensity of the cell corn of the PC - 3 cell. The result of being measured by Annexin V/PI staining indicated that the rate of apoptosis of PC - 3 tumor cell in vitro enhanced as the concentration of LA increased that showed dose - effect relationships. Conclusion ： The LA could inhibit the growth of many kinds of tumors in vitro, and LA could conform apoptosis in tumor cells as its one of the mechanism.

关 键 词：落叶松脂醇 体外 抗肿瘤作用 凋亡 

分 类 号：R285.5[医药卫生—中药学]