秀丽隐杆线虫脂肪染色的方法比较(英文)  

Using Different Dyes to Label Storage Fat in C.elegans

作　　者：冯婉娟[1] 黄文明[1] 许想平[1] 吴政星[1] 

机构地区：[1]华中科技大学生命科学与技术学院,中国湖北武汉430074

出　　处：《生命科学研究》2012年第1期9-14,共6页Life Science Research

基　　金：National Program on Key Basic Research Projec(t2006CB910701)~~

摘　　要：肥胖及其相关的疾病影响了越来越多的人.在哺乳动物中调节脂肪代谢的因子和脂肪代谢途径在线虫中也是保守存在的,因此线虫是脂肪研究的良好动物模型.在研究线虫脂肪代谢中,存在多种脂肪染色方法,而不同方法所表征的脂肪含量存在一定的差异,通过各种染色方法检测基因突变或者饥饿引起的脂肪含量变化.实验结果表明,采用油红、苏丹黑、尼罗红染料的固定染色法均能够正确地表征线虫的贮存脂肪.Obesity and its related diseases are affecting an increasing number of people. Many key fat regu- latory genes and fat metabolism pathways found in mammals are conserved in C. elegans. Therefore, C. ele- gans is a powerful genetic model for fat biology research. Different methods were used to quantitate the fat stores, and the results turn out different. According to the test in fixed worms during fasting or gene mutation, the dye such as Oil Red O, Sudan Black B, Nile Red and C 1-BODIPY-C 12 could quantitate fat stores correctly. Key words： C. elegans; fat stores; fluorescence dyes; Oil Red O; Sudan Black B

关 键 词：线虫 脂肪染色 荧光染料 油红 苏丹黑 

分 类 号：Q504[生物学—生物化学]