HPLC法测定杨梅叶中杨梅苷的含量  

Determination of Myricitrin in Leaves of Myrica rubra Sieb. et Zucc. by HPLC

作　　者：齐洁[1] 万竹青[1] 李默影[1] 吴秋月[1] 刘丽芳[1] 

机构地区：[1]中国药科大学天然药物活性物质与功能国家重点实验室,江苏南京210009

出　　处：《中国野生植物资源》2012年第1期35-37,共3页Chinese Wild Plant Resources

摘　　要：目的:建立HPLC法测定杨梅叶中杨梅苷含量的方法。方法:采用Diamonsil C18(150 mm×4.6 mm,5μm)柱,流动相乙腈-0.1%磷酸水溶液梯度洗脱,流速1.0 mL/min,检测波长358 nm,柱温30℃。结果:杨梅苷在0.088-0.880 mg/mL内与峰面积呈良好线性关系,A=36113C-401.56,r=0.9994;平均回收率为102.4%,RSD=1.9%(n=9)。测得杨梅叶中杨梅苷含量为1.43%。结论:该测定方法准确,可靠,对于杨梅的进一步开发利用具有重要的参考意义。To develop an HPLC method for determination of myricitrin in leaves of Myrica rubra Sieb. et Zucc.. Methods： The samples were separated on a Diamonsil Cl8 （150 mm×4.6 mm,5 ixm） column with the mobile phase of aeetonitrile - 0.1% phosphoric acid （ gradient elution）. The flow rate was 1.0mL/min. The detection wavelength was 358 nm. The column temperature was 30 ℃. Results： The linear range of myricitrin was 0.088 -0.880mg/mL, A =36113C -401.56, r =0. 9994. The average recovery was 102.4%, RSD = 1.9% （ n = 9 ）. The content of myricitrin in leaves of Myrica rubra Sieb. et Zucc. was 1.43%. Conclusion： The method was accurate and reliable, which is useful for the further exploring of this plant.

关 键 词：高效液相色谱法 杨梅叶 杨梅苷 

分 类 号：R284.1[医药卫生—中药学]