红花石榴二氢黄酮醇还原酶(DFR)基因cDNA片段克隆及序列分析  

Cloning and Sequence Analysis of cDNA Fragment of DFR Gene from Red-flower Pomegranate

作　　者：招雪晴[1] 苑兆和[1] 陶吉寒[1] 尹艳雷[1] 冯立娟[1] 

机构地区：[1]山东省果树研究所,山东泰安271000

出　　处：《山东农业科学》2012年第2期1-4,共4页Shandong Agricultural Sciences

基　　金：山东省自然基金项目(ZR2009DM010);国家外专局项目[鲁外专(2010)18号];山东省农业科技成果转化项目[鲁科农字(2010)79号]资助

摘　　要：二氢黄酮醇还原酶(DFR)是植物花色苷生物合成途径中的关键酶。以红花石榴"泰山红"的花瓣总RNA为模板,利用简并引物,采用RT-PCR方法,扩增出一条长446 bp的cDNA片段,序列分析结果表明,该cDNA片段与GenBank中的甜樱桃、葡萄等物种的DFR氨基酸同源性为78%～81%,说明该二氢黄酮醇还原酶片段为石榴花DFR基因的cDNA片段,GenBank登录号为JN316028。Dihydroflavonol 4-reductase（DFR）is the key enzyme in the biosynthesis of anthocyanidins.With the total RNA from red-flower pomegranate ＂Taishanhong＂ as template and using degenerate primers,a new cDNA fragment of 446 bp was amplified by RT-PCR.The sequence analysis demonstrated that the deduced amino acid sequence shared 78%～81% homology with that of sweet cherry,grape and etc.These results suggested that the cloned cDNA fragment was from DFR gene of red-flower pomegranate,and its accession number in GenBank was JN316028.

关 键 词：石榴 DFR基因 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]