EDS-40玻璃化液冻存小鼠4细胞胚胎的研究  

Study on the cryopreservation of mouses 4 cell embryo by the EDS-40 vitrification

作　　者：鲁栋梁[1] 崔曙[1] 陈在贤[2] 

机构地区：[1]川北医学院附属医院泌尿外科,四川南充637000 [2]重庆医科大学附属第一医院泌尿外科,重庆400016

出　　处：《四川医学》2012年第2期198-200,共3页Sichuan Medical Journal

基　　金：重庆市卫生局科研课题资助项目(编号:01-2-032)

摘　　要：目的研究玻璃化液EDS-40对冻贮小鼠4细胞胚胎的效果。方法①EFS-40和EDS-40玻璃化溶液的玻璃化测试。②随机将4细胞小鼠胚胎,在25℃室温下分别加入EDS-40和EFS-40玻璃化溶液,玻璃化后装管并迅速投入液氮中。对照组置于程序冷冻仪中进行程序化冷冻。各组均冻存3个月后,在25℃的水浴中复温后,用培养液反复洗涤后移入培养孔板培养,观察胚胎存活率及卵裂率。结果两种玻璃化溶液能达到玻璃化效果;EFS-40、EDS-40及对照组冷冻复温后的存活率分别87.8%、93.47%、80.77%;EFS-40、EDS-40及对照组冻胚的卵裂率分别:79.83%、86.53%、69.23%。结论 EDS-40玻璃化液冻贮小鼠4细胞胚胎的效果明显优于EFS-40,是一种理想的玻璃化液。Objective To study the cryoprotective effect of the solution of EDS-40 on mouse 4 cell embryo.Methods First test the verifying effect of EDS-40 and EFS-40 solutions,Then random chose 4 cell embryos of female mice to vitrify in EFS-40 or EDS-40 solutions at 25℃ and immerse into liquid nitrogen.Control group embryos were placed in progrom freezing equipment.All samples were cryopreserved 3 months.The embryos were rewarmed rapidly in 25℃ water,and agitated gently,then embryos were transferred into cultural medium and cultured.Then watch the survival rates and the merogenic rates.Results Both EDS-40 and EFS-40 solutions had vitrifying effects.The survival rates of EFS-40.EDS-40 and control group were 87.8%、 93.47%and 80.77% respectively;the merogenic rates of EFS-40、EDS-40 and control groups were79.83%、 86.53%and 69.23% respectivily.Conclusion The effect of EDS-40 solution is better than that of EFS-40.

关 键 词：玻璃化技术 玻璃化溶液 胚胎冻贮 

分 类 号：R714.8[医药卫生—妇产科学]