不同针刺时间对兔骨关节炎软骨细胞MMP-13的影响  

Effect of acupuncture in different period on the expression of MMP-13 in cartilage cells in the knee of rabbit osteoarthritis models

作　　者：蹇睿[1] 胥方元[1] 李卫平[1] 

机构地区：[1]泸州医学院附属医院康复科,四川泸州646000

出　　处：《海南医学》2012年第5期17-19,共3页Hainan Medical Journal

摘　　要：目的研究不同针刺时间对兔骨关节炎模型膝软骨细胞中MMP-13表达的影响。方法取44只新西兰大耳兔随机分为4组,分别为正常对照组,模型组及电针1组、电针2组。正常对照组不造模,模型组和电针治疗组均采用左膝关节伸直位管型石膏固定,建立OA模型。取兔左股骨内侧髁软骨,采用免疫组化法观察软骨细胞中MMP-13的变化。结果 MMP-13检出率随针刺时间延长较模型组下降。按Mankin's评分比较,各组间差异有统计学意义(P<0.05)。结论不同疗程电针治疗可促使兔骨关节炎模型软骨细胞重排,减少软骨细胞中MMP-13表达,对OA治疗有一定作用。Objective To study the effect of acupuncture in different period on the expression of MMP-13 in cartilage cells in the knee of rabbit osteoarthritis models. Methods Forty-four New Zealand big ear rabbits were randomly divided into four groups （each with 11 cases）： the control group, the model group, Electroacupuncture （EA） group 1, and EA group 2. Rabbit in the model group, the EA group 1 and EA group 2 were treated with straight bit left knee plaster casts to create osteoarthritis, while those in the control group did not. The changes of MMP-13 in cartilage cells were detected by immunohistochemistry. Results The levels of of MMP-13 decreased as the increase of acupuncture time. According to the Mankin＇s grading, statistically significant difference were found between the four groups （P0.05）. Conclusion The electroacupuncture can urge the rearrangement of cartilage cell in rabbit osteoarthritis models, reduce the expression of MMP-13 in cartilage cells, which is beneficial for the treatment of Osteoarthritis.

关 键 词：电针 骨关节炎 软骨细胞 

分 类 号：R684.3[医药卫生—骨科学]