3个水稻叶色突变体的遗传分析及基因定位  

Genetic analysis and mapping of three rice mutants with yellow leaf trait

作　　者：纪现军[1,2] 叶胜海[2] 周涯[2,3] 修芬连[2,3] 邓晓梅[1,2] 尚海漩[2,3] 刘继云[2,3] 陈萍萍[2] 金庆生[2] 张小明[2] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036 [2]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021 [3]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《浙江农业学报》2012年第1期7-11,共5页Acta Agriculturae Zhejiangensis

基　　金：浙江省科技厅项目(2010R50033;2008C22068);国家自然科学基金(31071207)

摘　　要：利用甲基磺酸乙酯对常规粳稻‘秀水09’进行诱变处理,获得了3个黄叶突变体Yl-1,Yl-2和Yl-3,多代自交稳定遗传。突变体与野生型‘秀水09’相比,分蘖减少、植株变矮、苗期叶片表现明显的黄色,随着生长发育的进行,叶片逐渐返绿。遗传分析表明这3个突变体的黄叶性状均受1对隐性核基因控制。利用SSR分子标记,将突变基因Yl-1定位在第3号染色体RM282与RM6080之间的7.5 cM范围内,将突变基因Yl-2和Yl-3定位在第5号染色体分子标记5-43w与RM1237之间,遗传距离分别为2.0 cM和6.8 cM。Three stably inherited yellow leaf mutants, YI-1, Y1-2 and Y1-3, were isolated from a japonica rice variety Xiushui 09 treated by ethyl methylsulfonate （EMS）. Compared with their wild type Xiushui 09, these mutants showed less tiller and shorter culm, and yellow leaves were observed at the seedling and tilling stages. With the growth of the mutant plants, the leaves turn green. Genetic analysis showed that the yellow leaf traits of the three mu- tants were all controlled by one pair of recessive nuclear genes. Genetic mapping of the mutant genes were conducted using yellow leaf individuals from the F2 mapping populations of YI-1, Y1-2 and Y1-3 crossed with indica variety Zhenshan 97. Y/-1 was mapped between SSR .markers RM282 and RM6080, within genetic distance of 7.5 cM in chromosome 3. While Y/-2 and I//-3 were mapped in the same site of chromosome 5 between 5-43w and RM1237, with genetic distances of 2. 0 cM and 6. 8 cM to each of them, respectively.

关 键 词：水稻 黄叶 遗传分析 分子定位 

分 类 号：S511[农业科学—作物学]