浙江地区不同寄主来源的炭疽菌ITS序列PCR-RFLP多态性分析  

Polymorphism analysis of Colletotrichum spp. from Zhejiang Province by ITS rDNA PCR-RFLP

作　　者：刘诗胤[1,2] 邱海萍[1] 姜华[1] 张震[1] 柴荣耀[1] 毛雪琴[1] 王教瑜[1] 杜新法[1] 王艳丽[1] 孙国昌[1] 

机构地区：[1]浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地浙江省农业科学院植物保护与微生物研究所,浙江杭州310021 [2]杭州师范大学生命与环境科学学院,浙江杭州310036

出　　处：《浙江农业学报》2012年第1期61-65,共5页Acta Agriculturae Zhejiangensis

基　　金：国家863计划子项;浙江省农业科学院创新提升项目

摘　　要：应用核糖体DNA(ribo-somal DNAr,DNA)的ITS(internal transcribed spacer,ITS)区的RFLP序列的多态性,分析32个不同寄主来源的炭疽菌株的遗传多样性。利用ITS1及ITS4通用引物对扩增各菌株的rD-NA的内转录间隔区(ITS1-5.8S-ITS2)获得长约600 bp片段。ITS-RFLP共迁带UPGMA聚类分析的结果表明所有来自不同寄主的炭疽菌菌株(含福建及云南菌株)都以较高的相似系数(>0.60)聚为一类,具有较近的亲缘关系;同时试验中所有酶切位点的综合Gst系数达到0.82,说明不同菌株间的ITS具有丰富的多态性。Colletotrichum spp. is the most common fungi in Zhejiang Province as well as the most important plant pathogens. There are so many kinds of species that it is important to find a way to identify and classify these fungi. Genetic diversity among 32 strains belonging to Colletotrichum spp. was assessed by RFLP analysis on the basis of rDNA ITS region. The PCR amplified ITS region was about 600 bp in length in all the tested strains and RFLP pat- terns of ITS products digested with different endonucleases （ Alu I , BsuR I , Hha I , Hpa ]] and Taq I ） were ana- lyzed. The UPGMA phenogram constructed with the pool of the ITS PCR-RFLP data revealed that all the isolates were clustered on one big group when the value of genetic similarity was 0. 6. The Gst parameter of all the ITS PCR- RFLP polymorphic bands was 0. 82 which indicated there were high diversity in the ITS region of all the isolates.

关 键 词：炭疽菌 RDNA 内转录间隔区 PCR-RELP 亲缘关系 

分 类 号：S432.1[农业科学—植物病理学]