异种脐血干细胞对胶原诱导关节炎小鼠滑膜组织形态结构影响的研究  

Infuence of heterogenous cord blood stem cell on the morphosis of synovial membrane histopathological in model mice with arthritis induced by collagen

作　　者：牛广华[1] 高玉洁[1] 高明利[1] 郭鹤[1] 明彩荣[1] 严峰[1] 

机构地区：[1]辽宁中医药大学附属医院,沈阳110032

出　　处：《国际检验医学杂志》2012年第4期385-387,共3页International Journal of Laboratory Medicine

基　　金：辽宁省自然基金项目资助(编号:20092033)

摘　　要：目的探讨异种、异基因脐血干细胞(HMSCs)移植对小鼠Ⅱ型胶原性关节炎(CIA)滑膜组织病理学的影响。方法 (1)应用美国BD公司FAcscaliburTM型分选式细胞分析仪,进行脐血CD34+细胞分选。(2)弗氏完全佐剂+Ⅱ型胶原诱导CIA小鼠模型,随机分为正常对照组、模型组、单份HMSCs移植治疗组、双份HMSCs移植治疗组、甲氨喋呤治疗阳性对照组,每组10只小鼠。(3)采用尾静脉注射,除模型组、正常对照组用生理盐水,单份、双份人脐血所含的CD34+细胞数均为2×105。(4)观察42d不同分组小鼠的关节症状及进行关节炎指数测定。(5)移植后第42天全部处死动物取踝关节进行病理组织学检测,收集数据并作统计分析。结果双份HMSCs移植优于单份HMSCs移植治疗组,差异有统计学意义(P<0.05)。结论双份HMSCs可作为一种新的替代细胞来源用于自身免疫性疾病的细胞移植治疗。Objective To observe histopathological changes of synovial membrane in type Ⅱ collagen-induced arthritis（CIA） modle mice, treated with transplantation of heterogenous allogeneic cord blood stem cells（HMSCs）. Methods （1）CD34 ＋ cord blood stem cells were selected and identificated with FAcscaliburTM flow cytometry sorting system of BD company. （2） CIA modle mice were induced by Freund＇s adjuvant and type [/collagen. All 50 mice were divided into 5 groups,including normal group,model group,mono-MSCs group, double-MSCs group and MTX group（10 mice for each group）. （3）Mice in mono-MSCs and double-MSCs group were injected with MSCs, with CD34 ~ cells for 2 × 10s , through caudal vein, but normal group and model group were infec- ted instead with 0.9 % normal saline. （4）Arthritis symptoms,osteoarthritis index and joint swelling degree of rheumatoid arthritis were observed for 42d in each group. （5）The histopathological change of synovial membrane on ankle joint was observed on the for- ty--second day,and the data was collected and analyzed. Results Double-MSCs transplantation was superior to mono-MSCs （P〈 0.05）. Conclusion Double-MSCs could be a new source of cell transplantation for the therapy of auto-immune disease.

关 键 词：关节炎 类风湿 胶原 滑膜 脐血干细胞移植 

分 类 号：R593.22[医药卫生—内科学]