枯草芽孢杆菌B-903菌株的研究 Ⅱ.抗菌物质的理化特性  

Study on Bacillus subtilis B 903 Strain Ⅱ.Characteristics of the Antifungal Substance

作　　者：孔建[1] 王文夕[1] 赵白鸽[1] 申效诚[1] 

机构地区：[1]河南省农科院植物保护研究所,郑州450002

出　　处：《中国生物防治》2000年第1期12-14,共3页Chinese Journal of Biological Control

基　　金：河南省农科院基础研究项目

摘　　要：枯草芽孢杆菌 B- 90 3菌株产生的抗菌物质 ,在偏酸性条件下能经受 1 2 1℃ (1 .4Pa/cm2 )高温 2 0分钟 ,仍保持原来的抗菌活性 ;在中性和偏碱性环境中不具备热稳定性。该抗菌物质在p H=4的溶液中 ,半年以上仍保持活性 ;但在碱性条件下 2个月即失活性 ,显示了其酸稳定性。在强酸 p H2～ 3条件下 ,可使有效物质从溶液中沉淀分离出来 ;调高 p H值又可使其重新溶于水中。Experiments showed that the antagonistic substance of B 903 strain still preserved it′s inhibitory action to pathogenic fungi when autoclaved at 121℃, (1 4Pa/cm 2) for 20 min in acid solution. However, this substance was not stable when treated by the same temperature at pH≥7.0. The antagonistic substance in acid solution (pH=4) could perserve it′s inhibiting function for more than 6 monthes but only for 2 monthes in an alkaline solution. This substance could be precipitated and isolated in an strong acid solution and dissolved again in alkaline solution.

关 键 词：枯草芽杆菌 抗菌素 理化性质 B-903菌株 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治] Q939.124[农业科学—植物保护]