nodD基因的克隆及其导入大豆根瘤菌工程菌株的构建  

Construction of genetic engineering strain of introduction extra nodD into native Rhizobium japonicum

作　　者：刘金玲[1,2] 张喜波[1,2] 贾珊珊[1,2] 平原[1,2] 李海英[1,2] 

机构地区：[1]黑龙江大学生命科学学院黑龙江省普通高等学校分子生物学重点实验室,哈尔滨150080 [2]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500

出　　处：《黑龙江大学自然科学学报》2012年第1期121-123,129,共4页Journal of Natural Science of Heilongjiang University

基　　金：哈尔滨市科技创新人才研究专项资金资助项目(RC2009XK001004);黑龙江省科技攻关项目(GA08B101);黑龙江大学高层次人才(创新团队)支持计划资助项目(Hdtd2010-05)

摘　　要：以快生型大豆根瘤菌(Sinorhizobium fredii)15067的基因组DNA作为模板,采用PCR技术,克隆结瘤基因nodD编码区序列;利用DNA重组技术,将nodD基因连到lac启动子的下游,通过luxAB基因标记的质粒载体pTR102构建表达载体pTR102-Plac-nodD,采用三亲本杂交的方式,将构建的表达载体转化土著大豆根瘤菌,构建转基因工程菌株,为研究转基因工程菌株对大豆结瘤能力的影响提供依据。PCR technology was used to clone nodulation gene nodD with template of genomic DNA from Sinorhizobium fredii 15067 in this study. Utilization of DNA recombination technique, nodD gene was joined into downstream of lac promoter. And expression vector pTRlO2-Plac-nodD was constructed by using plasmid pTR102 marked by luxAB gene. The expression vector pTRlO2-Plac-nodD was transformeded into native Rhizobiurn japonicum with method of triparental hybridization to construct genetic engineering strain. It will lay a foundation for the study of genetic engineering strain influence on nodulation ability of glycine max.

关 键 词：大豆根瘤菌 nodD基因 工程菌株 三亲本杂交 

分 类 号：S566.3[农业科学—作物学] S188