甜菜M14品系Cystatin基因植物蛋白纯化表达载体的构建及转化烟草  

Construction of plant purification vector of BvM14-Cystatin and transformation of tobacco

作　　者：王宇光[1,2] 詹亚男[1,2] 宫世龙[1,2] 于冰[1,2] 杨乐[1,2] 陈思学[1,2] 李海英[1,2] 

机构地区：[1]黑龙江大学生命科学学院黑龙江省普通高等学校分子生物学重点实验室,哈尔滨150080 [2]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500

出　　处：《黑龙江大学自然科学学报》2012年第1期135-140,共6页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金资助项目(31071473;30871566);黑龙江大学高层次人才(创新团队)支持计划资助项目(Hdtd2010-05)

摘　　要：植物半胱氨酸蛋白酶抑制剂是一类具有提高植物对各种生物及非生物胁迫抗逆性的重要蛋白质。利用前期获得的甜菜M14品系半胱氨酸蛋白酶抑制剂基因的编码区序列构建了带有His标签的甜菜M14品系半胱氨酸蛋白酶抑制剂基因的植物蛋白纯化表达载体,并将其转化烟草,获得T0代阳性转基因植株,为下一步利用融合蛋白的His标签从转基因烟草中大量纯化甜菜M14品系半胱氨酸蛋白酶抑制剂奠定了基础。It has been found that phytocystatin could improve crops ability of disease resistance and reduce the abiotic injury. The eukaryotic purification vector of the BvM14-cystatin had been constructed, which will express a His-Taged BvM14-Cystatin in plants. Moreover, some TO generation transgenic tobaccos were selected for the purification of BvM14-Cystatin through affinity chromatography in a nickel column. This research established a solid foundation for purifying the BvM14-cystatin protein with the expression system of tobacco protein, and the characteristic of this protein is studied.

关 键 词：甜菜M14品系 半胱氨酸蛋白酶抑制剂 转化 

分 类 号：S566.3[农业科学—作物学]