濒危药用植物天山雪莲多倍体植株的化学诱导  

Polyploid Induction of Saussurea involucrata an Endangered Chinese Medicinal Herb

作　　者：赵红艳[1] 江丽丽[2] 马淼[2] 

机构地区：[1]新疆师范大学生命科学学院,乌鲁木齐830054 [2]石河子大学生命科学学院,新疆石河子832003

出　　处：《种子》2012年第2期21-24,30,共5页Seed

基　　金：国家自然科学基金项目(项目编号:30960028);新疆师范大学优秀青年教师科研启动基金项目(项目编号:XJNU 0727)联合资助

摘　　要：以叶片诱导的愈伤组织为材料,用秋水仙素溶液浸泡法和秋水仙素加入培养基法分别进行诱导。实验结果表明,秋水仙素加入培养基法要好于秋水仙素溶液浸泡法,具体表现为:用秋水仙素加入培养基法诱导出的愈伤组织褐化死亡率低,分化率高,四倍体细胞平均比例高。而在处理浓度与时间的选择中,以高浓度的秋水仙素与短时间处理组合较好。秋水仙素的浓度为600 mg/L处理3 d效果最佳,四倍体细胞比例达41.5%。Callus induction from leaf explant was disposed in two methods：immersion in colchicine solution;adding the colchicine in the medium.The result showed that when operated the callus by the method of adding the colchicine in the medium,the browning rate and mortality were low,the differentiation rate was high,and the proportion of tetraploid cell was high,which was better than the method of immersion in colchicine.Meanwhile,the treatment combination of high concentrations of colchicine and short time was the best.41.5% tetraploid cell proportion was got when added 600 mg/L colchicine in medium and continuous cultured 3 days.

关 键 词：天山雪莲 愈伤组织 多倍体 嵌合体 濒危植物 

分 类 号：S567[农业科学—中草药栽培]