pcDNA3．1（＋）载体携带血管内皮生长因子治疗大鼠缺血皮瓣  

Effect of pcDNA3. 1 （ ＋ ） .mediated vascular endothelial growth factor gene on the survival of skin flap

作　　者：茹靖涛[1] 曹靖[2] 秦涛[3] 

机构地区：[1]河南省人民医院急诊创伤外科,郑州450003 [2]郑州大学基础医学院 [3]河南省人民医院器官移植中心,郑州450003

出　　处：《中华实验外科杂志》2012年第3期424-426,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（31140078）

摘　　要：目的观察注射真核表达载体pcDNA3．1（＋）携带血管内皮生长因子（VEGF）基因后对大鼠缺血皮瓣存活的影响。方法构建重组质粒pcDNA3．1（＋）一VEGF，将sD大鼠随机分成3组，每组10只。pcDNA3．1（＋）-VEGF组：于背部皮肤皮下多点注射pcDNA3．1（＋）-VEGF30μg／100μl；VEGF组：多点注射VEGF蛋白100ng／100μl；生理盐水（Ns）组：注射生理盐水100μl，注射2d后在其背部形成7cm×3cm的全厚随意型皮瓣，48h后按原设计掀起皮瓣并原位缝合。术后10d测量皮瓣的成活面积，计算成活面积百分比，并行免疫组织化学检测。结果皮瓣成活面积百分比：pcDNA3．1（＋）-VEGF组为（79．1±3．5）％，VEGF组为（62．2±2．7）％，NS组为（59．9±3．1）％，pcDNA3．1（＋）-VEGF组皮瓣成活面积明显高于其他两组（P〈0．05）。结论皮下注射pcDNA3．1（＋）-VEGF可促进新生血管的形成，比单纯注射VEGF蛋白更能提高皮瓣的成活率。Objective To investigate the effectiveness of PeDNA3. 1 （ ＋ ）-mediated gene therapy of vascular endothelial growth factor （VEGF） delivered into the subcutaneous space to treat experimental skin flaps in rats. Methods Thirty Spragne-Dawley rats were randomly divided into three groups （ n = I0 each）. In pcDNA3. 1 （ ＋ ）-VEGF group, rats were given subcutaneous injections of pcDNA3. I （ ＋ ）- VEGF 30 μg/100 μL; In VEGF group, rats were given subcutaneous injections of VEGF 100 ng/100μL; In NS group, rats were given subcutaneous injections of normal saline. Pcdicled flap （7 cm ＋ 3 cm） of full thickness was made on rats back in each group 48 h after subcutaneous injections. The survival rate was measured and immunohistoehemieal examinations were done 10 days after operation. Results The survival rate of the flap in pcDNA3. 1-VEGF group was increased significantly as compared with the other two groups （P 〈0. 01 ）. Immunohistochemical staining showed that VEGF was highly expressed in the pcDNA3. 1 （ ＋ ）-VEGF group. Conclusion Subcutaneous injections of pcDNA3. 1 （ ＋ ）-VEGF efficiently improve the survival of ischemic skin flap.

关 键 词：血管内皮生长因子 皮瓣 基因治疗 

分 类 号：R6[医药卫生—外科学]