蛋白质谱技术预测大鼠心脏移植后急性排斥反应  

Prediction of acute heart transplant rejection in rats with protein spectrum technique

作　　者：王广阔[1] 林峰[1] 叶韵斌[2] 陈慧菁[2] 黄雪珊[1] 陈道中[1] 

机构地区：[1]福建医科大学附属协和医院心外科,福州350001 [2]福建省肿瘤医院肿瘤免疫学研究室福建省肿瘤转化医学重点实验室福建省肿瘤转化医学重点实验室

出　　处：《中华实验外科杂志》2012年第3期491-494,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨蛋白质谱技术对大鼠心脏移植后急性排斥反应的预测。方法建立颈部异位异种大鼠心脏移植模型。（1）急性排斥A和B组，供受体不作预处理。（2）治疗A和B组，受体术前1d腹腔注射环孢素A（CsA）20mg／kg，术后每天10mg／kg。A和B组分别在术后5d和7d取血液标本及移植物（供心）标本。（3）空白组，取大鼠移植前血液及心肌组织作标本。应用蛋白质谱技术对各组的血清及心肌组织蛋白质样本进行质谱测定，统计分析各组之间的差异蛋白质，并比较分析差异蛋白质的峰强度。结果与空白组血清比较，急性排斥A组与之存在7个差异蛋白质峰，急性排斥B组与之存在11个差异蛋白质峰。急性排斥A组与治疗A组比较，有4个差异蛋白质峰，急性排斥B组与治疗B组比较，有6个差异蛋白质峰。与空白组心肌组织蛋白质比较，急性排斥A和B组与之分别存在7个和13个差异蛋白质峰。以上比较差异均有统计学意义（P〈0．01）。结论正常与急性排斥反应后不同时相点血清及心肌组织中的蛋白存在明显差异，这种差异蛋白可能与急性排斥反应相关。Objective To study the prediction of acute heart transplant rejeetiQn in rats with pro- tein spectrum technique. Methods The xenogeneic rat cervical heterotopic heart transplantation model was set up. （ 1 ） The donors and recipients of acute rejection groups A and B were not subjected to pre-treat- ment; （2） In treatment groups A and B, receptors were subjected to intraperitoneal injection of cycl0spo- rine A （CsA） 20 mg/kg one day preoperation, 10 mg/kg per day postoperation. At 5th and 7th day posto- peration, blood samples and the graft （donor hearts） specimens were collected; （3） In control group, blood and myocardial tissue specimens were taken from the healthy Wistar rats before transplantation. By using proteomic technologies, serum samples and the myocardial tissue samples of protein in all groups were measured. The differential proteins among group were analyzed and the peak intensity of the differenti- al proteins was compared. Results As compared with the serum samples of control group, there were 7 differential protein peaks in acute rejection group A, and 11 differential protein peaks in acute rejection group B. The comparison between acute rejection group A and treatment group A revealed that there were 4 differential protein peaks. The comparison between acute rejection group B and treatment group B showed that there were 6 differential protein peaks. For myocardial proteins in the control group compared to acute rejection groups A and B, there were 7 and 13 differential protein peaks, respectively. Above comparisons were statistically significant （P 〈 0. 01 ）. Conclusion Differential proteins in acute rejection at different time points exist, wich may be related with acute rejection.

关 键 词：心脏移植 急性排斥 蛋白质谱技术 

分 类 号：R65[医药卫生—外科学]