荧光定量PCR技术检测乳腺癌患者外周血EGFRmRNA及CK20mRNA表达的意义  

The study of detection of epidermal growth factor receptor and cytokeratin-20mRNA in peripheral blood of primary breast cancer patients by fluorescence quantitative polymerase chain reaction

作　　者：弓自玉[1] 熊兵红[2] 马利[3] 

机构地区：[1]四川省广元市精神卫生中心,广元628001 [2]重庆医科大学附属第一医院,重庆400016 [3]重庆市巴南区第二人民医院,重庆400054

出　　处：《按摩与康复医学》2012年第5期22-23,共2页Chinese Manipulation and Rehabilitation Medicine

摘　　要：的：检测乳腺癌患者外周血中表皮生长因子受体（epidermal growth factor receptor,EGFR）及细胞角蛋白20（cytokeratin20 CK20）并分析其临床病理联系.方法：应用荧光定量PCR（FQ-PCR）检测46例乳腺癌及30例女性健康人外周血CK20 mRNA及EGFRmRNA.结果：30例健康对照组外周血CK20mRNA及EGFRmRNA均为阴性.患者组EGFR及CK20mRNA表达阳性分别为47.83% （24/46）,21.74% （10/46）.EGFR mRNA及CK20mRNA表达阳性在TNM分期中分别为Ⅰ期（2/6,1/6）,Ⅱ期（4/13,3/13）,Ш期（12/19,11/19）及IV期（4/8,3/8）,（P〈0.05,Ⅰ＋Ⅱ期与Ш＋Ⅳ期）;与对照组比较有显著差异.结论：FQ-PCR方法检测乳腺癌患者外周血中EGFR mRNA、CK20mRNA表达与其临床及病理分期相关.此两项指标联合检测可作为乳腺癌诊断、治疗及预后的参考指标.Objective：To explore the eytokerafin 20 （CK20） and epidermal growth factor receptor（EGFR） in peripheral blood of the patients with breast cancer and evaluated the clinical significance. Methods：The expression of EGFRmRNA and CK20mRNA in peripheral blood was examined by fluorescence quantitative-polymerase chain reaction（FQ-PCR） in 46 patients with breast canc- er and30 normal controls. Results：In peripheral blood from 30 healthy donors, neither EGFRmRNA nor CK20mRNA was expressed. The EGFRmRNA atad CK20mRNA positive were detected in breast cancer patients [47.83M （24/46）,21.74% （10/46）1. The expression of EGFRmRNA and CK20mRNA in TNM clinical stage were found in [ （2/6,1/6 in breast cancer）, II （4/13,3/13in breast cancer）,III（12/19,11/19 in breast cancer ） and 1V （4/8,3/8 in breast cancer）, （ P 〈0.05, I , II vs III, IV ）, respectively. There were difference of the EGFR and CK20 expression rate hetween the patients in I and II and IIIand IV clinical stage. Conclusion：The expression of EGFRmRNA and CK20mRNA in peripheral blood of patients with breast cancer correlated with clinical stage. These data suggested that the detection of EGFR and CK20mRNA by using FQ-PCR are useful marker of circulating tumor cell and will he helpful in assessing prognosis,tailoring therapy,or developing new strategies.

关 键 词：乳腺癌 细胞角蛋白20 表皮生长因子受体 荧光定量PCR技术 

分 类 号：R4[医药卫生—临床医学]