红谷霉素对细菌耐药性质粒转移的抑制作用  

Inhibition Effect of Honggumycin on Drug Resistance R Plasmid Transfer Between Bacteria

作　　者：黎循航[1] 薛秀园[1] 张慧雯[1] 武琳[1] 倪国荣[1] 涂国全[1] 

机构地区：[1]江西农业大学生物科学与工程学院,南昌330045

出　　处：《应用与环境生物学报》2012年第1期153-156,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.31071724);江西省自然科学基金项目(No.2010GZN0037)资助~~

摘　　要：以Shigella flexneri D15 R-plasmid CmR和Escherichia coil 1485 RifR为试验菌株,研究红谷霉素对细菌耐药性质粒在细菌之间转移的抑制作用及对两种细菌R质粒的消除作用.将红谷霉素(30μg/mL)与S.flexneri D15 R-plasmid CmR和E.coli 1485 RifR按实验设计混合并分别培养24 h和48 h,涂布在麦康凯琼脂平板上,计算相应菌落数.结果表明,当红谷霉素抑菌浓度为30μg/mL时,对细菌耐氯霉素的R质粒的转移抑制率分别达到92.82%(24 h培养)和96.04%(48 h培养),但对R质粒的消除作用不显著.说明红谷霉素对R质粒转移抑制作用强烈但不具有R质粒的消除作用.Shigella flexneri D15 R-plasmid CmR and Escherichia coli 1485 RifR were used to investigate the possible inhibition influence of Honggumycin on conjugative drug resistance R plasmid transfer between bacteria and elimination of R plasmid from the objective bacteria.30 μg/mL of Honggumycin was mixed with S.flexneri D15 R-plasmid CmR or E.coli 1485 RifR,which were separately cultured for 24 h and 48 h on MacConkey agar with or without chloromycetin.The inhibition rate and elimination rate of R plasmid were calculated according to the number of colonies from test group and control group.The results indicated that the transfer rate of R plasmid reached 92.82% and 96.04% in the medium with 30 μg/mL of Honggumycin for 24 h and 48 h,respectively.Besides,there was no significant effect of Honggumycin on elimination of R plasmid from S.flexneri D15 R-plasmid CmR.Therefore,Honggumycin could effectively inhibite conjugative R plasmid transfer from S.flexneri D15 R-plasmid CmR to E.coli 1485 RifR and was impossible to eliminate R plasmid.

关 键 词：红谷霉素 R质粒 细菌耐药性 质粒转移 

分 类 号：R96[医药卫生—药理学] Q93[医药卫生—药学]