荞麦黄酮复方制剂对大鼠2型糖尿病心脏小血管病变的保护作用  

The Effect of the flavonoid of buckwheat compound preparation on cardiac small vessels pathological changes in type 2 diabetes rats

作　　者：姜妍[1] 赵慧芹[1] 王健行[1] 韩淑英[1] 

机构地区：[1]河北联合大学,河北唐山063000

出　　处：《中国现代医学杂志》2011年第30期3741-3745,共5页China Journal of Modern Medicine

基　　金：河北省自然科学基金资助项目(No:C2010001795)

摘　　要：目的探讨荞麦黄酮复方制剂(TFBCP)对2型糖尿病大鼠心脏小血管病变的保护作用及其可能机制,为2型糖尿病的临床康复治疗提供一些理论依据。方法将SD大鼠70只,随机取出12只作为正常对照组(Contorl group),其余通过高脂高热量饮食加小剂量链脲佐菌素腹腔注射,建立大鼠2型糖尿病模型。将成模大鼠随机分为4组,即TFBCP低、高剂量组(L、H-TFBCP group)、卡托普利组(Cap group)和模型对照组(Model group),分别灌胃给药0.6、1.2和0.5g/(kg·d),正常对照组和模型组每天灌服相同容积生理盐水,连续8周。观察空腹血糖(FBG)和光镜下心脏小血管组织结构变化。采用比色法测定血清甘油三酯(TG)、胆固醇(T-CHO)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、极低密度脂蛋白(VLDL);放射免疫分析法测定心脏小血管单核细胞趋化蛋白-1(MCP-1)的含量。以荧光光度计测定血清晚期糖基化终产物(AGEs)含量。采用Western-Blot法结合计算机图像分析技术,检测心脏小血管单核细胞趋化蛋白-1(MCP-1)表达的变化。结果 TFBCP能剂量依赖性降低2型糖尿病大鼠FBG、TG、T-CHO、LDL,VLDL,并可升高HDL含量。能改善心脏小血管的病理变化,对心脏小血管MCP-1蛋白表达上调有一定逆转作用,其中TFBCP高剂量组与阳性药物卡托普利效果相近。结论 TFBCP对2型糖尿病大鼠心脏小血管病变具有保护作用,其机制可能是拮抗了P38α-MAPK信号转导通路的MCP-1级联,亦可能是抑制了血浆AGEs含量。[Objective]To study the effects of The Flavonoid of Buckwheat Compound Preparation（TFBCP） on cardiac small vessels pathological changes in type 2 diabetes rats and its mechanism.[Methods]The SD rats were 70,12 randomly selected as a control group.The rests were given high-fat diet,then were stimulated by the ip of small dosage of Streptozocin to build diabetes model.The Type 2 Diabetes Mellitus rats were randomly divided into 4 groups-L、H-TFBCP group,Cap group and Model group by ig 0.6、1.2 and 0.5 g/（kg·d） for 8 weeks.Model group and control group were given equal volume sodium chloride.At the end of the experiment,the fasting blood glucose （FBG） was detected;triglyceride（TG）,total cholesterol（T-CHO）,high density lipoprotein（HDL）,low density lipoprotein （LDL）,very low density lipoprotein（VLDL） and monocyte chemoattractant protein-1（MCP-1） were measured. The changes of cardiac small vessels were observed under light microscope.The content of AGEs in serum was detected by spectrofluorometer.Western-Blot techniques were used to detect MCP-1 expression.[Results]TFBCP resulted in a dose-dependent decreased in FBG,TG,T-CHO,LDL and VLDL content,while HDL content increased markedly.And it can improve the small vessels pathologic changes.TFBCP intervention group could inhibit the MCP-1 expression.H-TFBCP group is more effective,the role and Cap group were similar.[Conclusion]TFBCP has some protective effects on Small vessels pathological changes in type 2 diabetes rats.The mechanism may be related to inhibition of MCP-1 cascade in P38a-MAPK signal transduction system.It also be related to the content of serum AGEs.

关 键 词：麦黄酮复方制剂 2型糖尿病 单核细胞趋化蛋白-1 WESTERN-BLOT 

分 类 号：R332[医药卫生—人体生理学]