Cbfα-1和MCP-1在DN大鼠肾内小动脉中表达意义及相关性研究  

Significance of detection on the expression of core-binding factor α1 and monocyte chemoattractant protein 1 in renal Arteriole of diabetic nephropathy rats

作　　者：赵安菊[1] 黄颂敏[2] 欧三桃[2] 王里[1] 陈超[1] 

机构地区：[1]贵州省人民医院干医科,贵州贵阳550002 [2]四川大学华西医院肾内科,四川成都610041

出　　处：《中国现代医学杂志》2011年第33期4109-4113,共5页China Journal of Modern Medicine

基　　金：贵州省科技厅基金(黔科合J字[2008]2299号);贵州省卫生厅优秀医学青年科技人才基金(gzwkj2010-2-006)

摘　　要：目的观察核心结合因子α亚单位1(Cbfα1)及单核细胞趋化蛋白1(MCP-1)在糖尿病肾病(DN)大鼠肾内小动脉上的表达及其变化规律,探讨其两者关系对糖尿病肾病血管病变的影响。方法设对照组、糖尿病肾病组两组,建立STZ诱导的DN大鼠模型。采用茜素红染色观察肾实质内小动脉周围钙盐沉积,分别采用免疫组化及mRNA原位杂交法检测Cbfα1和MCP-1在肾实质内小动脉上的蛋白表达及基因定位。方法①DN组各时间点血糖及尿蛋白量(24h)均较C组显著增高(P<0.05),自第12周开始,DN组血肌酐较C组显著增高(P<0.05)。②茜素红染色DN组第4～12周时偶见肾内小动脉少许点状红色钙盐沉积,第24周时可见肾实质内小动脉及周围组织有较多红色点片状沉积,C组茜素红染色为阴性。③DN组肾实质内小动脉Cbfα1蛋白及mRNA在4周时已有表达,随时间延长表达逐渐增强,各时间点均明显高于对照组,C组各时间点表达差异无统计学意义;DN组肾实质内小动脉MCP-1蛋白及mRNA在4、12周表达无显著差异,24周表达明显上调,C组肾小动脉无表达。④Cbfa1与MCP-1呈明显正相关关系(r=0,495,P<0.01)。结论①DN大鼠肾内小动脉钙化前即已有Cbfa1的表达;②炎症因子MCP-1可影响Cbfα1的表达;③肾内小动脉上Cbfa1、MCP-1的表达变化可能参与DN进展。[ Objective ] To observe the expressions of core-binding factor αl （Cbfαl） and monocyte chemoattrac- rant protein 1 （MCP-1） in the renal arteriole of diabetic nephropathy （DN） rats and further analyze their correlations with diabetic nephropathy. [Methods] Adult Sprague-Dawley male rats were divided into diabetic group （DN） and control group （C）. The animal model of diabetic nephropathy was established by peritoneal injection of 55 mg/kg of streptozoein. Calcium deposit around the renal arteriole was observed by alizarin red staining. The protein and mRNA levels of Cbfal and MCP-1 in renal arteriole of DN rats were detected by immunohistochemistry and in situ hybridization. [ Results ] ①Less deposit of calcium salt was observed in the renal arteriole of DN rats at 4 week while a large amount of deposit of calcium salt was observed at 24 weeks. No calcium deposit was observed in control rats. ②Cbfαl expression was significantly increased in the renal arteriole of DN rats from 4 to 24 weeks compared with the control rats. ③MCP-1 expression was obviously up-regulated in the renal arteriole of DN rats at 24 weeks compared with that at 4、12 weeks. No MCP-1 expression was observed in the renal arterioles of control rats. ④Cbfalexpression positively correlated with MCP-1 expression （r =0.495, P 〈0.01）. [ Conclusion ] ①Cbfαl has already expressed in renal arteriole before the formation of vascular calcification; ②MCP-1 can affect the expression of Cbfα1; ③Cbtαl and MCP-1 may play a role in the development of diabetic nephropathy.

关 键 词：糖尿病肾病 血管钙化 炎症因子 核心结合因子α亚单位1 

分 类 号：R587.2[医药卫生—内分泌]