双氯芬酸对脂多糖诱发脑内核团Fos表达的影响  

Effect of diclofenac sodium on fos expression in immunity correlative neurons induced by lipopolysaccharide in rat

作　　者：徐乃玉[1] 郭试瑜[2] 久光正[2] 

机构地区：[1]苏州大学医学部药学院,江苏苏州215123 [2]日本昭和大学医学部

出　　处：《中国现代医学杂志》2011年第34期4239-4243,共5页China Journal of Modern Medicine

摘　　要：目的观察双氯芬酸对脂多糖(LPS)免疫应激时大鼠下丘脑室旁核(PVN)、视上核(SON)及脑干蓝斑(LC)中Fos表达的影响。方法大鼠随机分成正常对照组、模型组、双氯芬酸实验组(5,20mg/kg),利用Fos蛋白、加压素(AVP)和酪氨酸羟化酶(TH)双重免疫组织化学方法,定位并检测上述核团中Fos阳性神经元的数目,并进行统计学分析。结果与对照组比较,腹腔注射LPS明显诱发PVN、SON和LC出现大量Fos样免疫反应神经元(P<0.01),Fos-AVP、Fos-TH双标神经元明显增加;双氯芬酸能剂量依赖地降低LPS诱导的Fos阳性神经元数目。结论双氯芬酸下调不同脑区LPS免疫应激Fos蛋白的表达与其抑制PGs的合成有关,通过下调下丘脑-垂体-肾上腺皮质(HPA)轴的活动,从而发挥中枢应激调节作用。[ Objective ] To observe the effect of diclofenac sodium on Fos expression in arginine vasopressin nueleus（AVP） in paraventricular nucleus （PVN）, supraoptic nucleus （SON） and tyrosine hydroxylase neurons（TH） in locus coeruleus （LC） of the rat induced by lipopolysaccharide. [ Methods] Wistar rats were divided randomly into NS group, model group, diclofenac sodium groups （5, 20mg/kg）. The changes in the number of los positive neurons in AVP nucleus and TH nucleus were detected by dual-labeling immunohistochemistry method. [Results] Compared with the control group, the number of the los-labeled neurons increased significantly in model group （P 〈0.01） and decreased in a dose-dependent manner in the diclofenac sodium groups. [ Conclusions] Down-regulation of los ex- pression in different regions of brain is related with the inhibition effect of the dielofenac sodium on the synthesis of prostaglandins （PGs）, which can regulate the nerve centre by alleviating the activation of HPA pathway.

关 键 词：脂多糖 加压素 酪氨酸羟化酶 FOS蛋白 双氯芬酸钠 

分 类 号：R966[医药卫生—药理学]