乙肝肝硬化患者血清乙肝病毒表面抗原定量水平的研究  

The quantitive level of Hepatitis B surface antigen in patients with HBV-related liver cirrhosis

作　　者：马晓艳[1] 韩涛[2] 裴彦祯[1] 李莹[2] 邢晶[1] 宋佐莉[3] 

机构地区：[1]天津医科大学三中心临床学院肝内科,天津300170 [2]天津市第三中心医院肝内科(天津市肝胆疾病研究所天津市人工细胞重点实验室),天津300170 [3]天津市第三中心医院检验科,天津300170

出　　处：《中国现代医学杂志》2011年第34期4275-4277,4281,共4页China Journal of Modern Medicine

基　　金：国家重点基础研究发展规划基金(973计划)(No:2007CB512801);"十一五"科技重大专项(No:2008ZX10002-005);天津市卫生局重点攻关项目(No:07KG9)

摘　　要：目的分析不同Child-pugh分级的乙肝肝硬化患者血清乙肝病毒表面抗原(HBsAg)定量检测水平及其在各组间与HBV DNA含量之间的相关性。方法收集乙肝肝硬化患者血清,其中Child-pugh A级38例,B级46例,C级35例,采用雅培化学发光法进行HBsAg定量测定,采用荧光PCR定量法检测HBV DNA含量。结果 HBsAg定量值分别为Child-pugh A级[997.70(146.00～1717 12)IU/mL],Child-pugh B级[1158.78(345.82～1889.93)IU/mL],Child-pugh C级[613.76(120.78～1528 77)IU/mL],三组比较差异无统计学意义(P=0.464)。HBsAg定量与HBV DNA相关性分析:HBsAg定量水平、HBV DNA含量在乙肝肝硬化患者总体中呈正相关(r=0.353,P=0.000),其中Child-pugh A级相关性最高(r=0.410,P=0.011),Child-pugh B级次之(r=0.366,P=0.012),Child-pugh C级则无相关性(r=0.293,P=0.088)。在各组中HBsAg定量水平与ALT、AST和TBIL均无相关性。结论不同Child-pugh分级的乙肝肝硬化患者之间血清HBsAg定量水平无差异;HBsAg定量水平与HBV DNA在Child-pugh A级、B级组具有正相关性,C级组无相关性。[Objective] To investigate HBsAg quantitive level in different Child-Pugh scores in patients with HBV-related liver cirrhosis （LC） and the correlation with HBV DNA level in each cohort. [ Methods ] HBsAg level and HBV DNA were measured among 38 Child-pugh A cohort, 46 Child-pugh B cohort, 35 Child-pugh C cohort using Abbott chemiluminescence and fluorescence quantitative PCR, respectively. [ Results ] The HBsAg quantitive level in serum was 997.70 IU/mL in the Child-pugh A cohort, 1158.78 IU/mL in the Child-pugh B cohort, 613.76 IU/mL in the Child-pugh C cohort respectively, and there were no statistical difference among these cohorts （P = 0.464）. Correlation analysis between HBsAg level and HBV DNA showed that they had a positive correlation in over- all （11 =0.353, P =0.000）, and the correlation was strongest in the Child-pugh A cohort （r =0.410, P =0.011）, and the correlation in Child-pugh B cohort second （r =0.366, P =0.012）, but no correlation was found in the Child-pugh C cohort （r =0.293, P =0.088）. There was no correlation between HBsAg level and ALT, AST, TBIL level in all cohorts. [ Conclusion ] The level of HBsAg quantity in serum has no difference among different Child-Pugh scores in patients with LC. The level of HBsAg quantity and HBV DNA have a positive correlation in Child-pugh A and B cohort, but no correlation is found in the Child-pugh C cohort.

关 键 词：Child—pugh分级 乙型 肝硬化 乙肝病毒表面抗原 乙肝病毒DNA 

分 类 号：R512.62[医药卫生—内科学] R575.2[医药卫生—临床医学]