胃癌患者腹腔冲洗液中CEAmRNA及HpamRNA表达对判断腹腔微转移的临床意义  

The clinical significance of the expression of CEAmRNA and DDCmRNA in the peritoneal lavage fluid in patients with gastric cancer for abdominal cavity micrometastases

作　　者：费建东[1] 聂双发[1] 郭飞[1] 梁峰[1] 胡晓峰[1] 安永铸[1] 

机构地区：[1]河北北方学院附属第一医院普外科,河北张家口075000

出　　处：《中国现代医学杂志》2011年第34期4282-4285,共4页China Journal of Modern Medicine

摘　　要：目的探讨腹腔冲洗液中CEAmRNA、HpamRNA及MMP-7mRNA表达情况与腹膜微转移关系。方法采用RT-PCR技术检测80例胃癌患者及16例胃良性肿瘤患者的腹腔冲洗液中的CEAmRNA、HpamRNA及MMP-7mRNA表达水平,并与传统的腹腔冲洗细胞学(PLC)对比分析,寻找与胃癌腹膜微转移有关的肿瘤标志物。结果胃癌组分别与胃良性瘤组患者比较,腹腔冲洗液中CEAmRNA、HpamRNA、MMP-7mRNA表达阳性率明显增高(P<0.05),其中CEAmRNA最高,其次为HpamRNA,MMP-7mRNA的阳性率较低(P<0.05);CEAmRNA、HpamRNA和MMP-7mRNA这三种指标在临床分期、组织学类型、pTNM、淋巴结转移及肝转移间阳性率比较,统计分析显示均有统计学意义(P<0.05),而在肿瘤部位方面,差异无统计学意义,随着临床分期的增加,这些肿瘤标志物的表达率也明显升高,有淋巴结及远处转移的患者较未转移的患者阳性率也明显升高;随着肿瘤浸润深度的增加,CEAmRNA、HpamRNA、MMP-7mRNA及PLC阳性率也明显增加,统计分析显示有统计学意义(P<0.01)。结论采用RT-PCR技术检测腹腔冲洗液中的CEAmRNA及HpamRNAA表达,可以用于预测胃癌患者腹膜微转移。[ Objective ] To evaluate the relationship of the expression of CEAmRNA, DDCmRNA and PSPHmRNA in the peritoneal lavage fluid and peritoneal metastasis in patients with gastric cancer. [ Methods ] RT-PCR was used to detect the expression levels of CEAmRNA, DDCmRNA and PSPHmRNA in the peritoneal fluid of 80 eases with gastric cancer and 16 patients with benign tumors, which was compared with the traditional peritoneal washing cytology （PLC） to search tumor markers related to gastric cancer peritoneal mierometastasis. [Results] Compared with benign gastric tumors group, the CEAmRNA, DDCmRNA, PSPHmRNA expression levels in the abdominal fluid of the gastric cancer group were significantly higher （P 〈0.05）, among which CEAmRNA was the highest, followed by DDCmRNA, and PSPHmRNA has the lowest positive rate （P 〈0.05）. The statistical analysis of positive rates of these three indicators, CEAmRNA, DDCmRNA and PSPHmRNA in the clinical stage, histological type, PTNM, lymph node metastasis and liver metastasis had statistical significance （P 〈0.05）, while in tumor, the difference was not sta- tistically significant, the expression levels of these tumor markers significantly increased with increasing clinical stage, the positive rate of these markers in patients with lymph node and distant metastases was significantly higher than that in patients without metastasis. With the increasing depth of tumor infiltration, CEAmRNA, DDCmRNA,PSPHmRNA and PLC positive rate also increased significantly, which showed statistical significance （P 〈0.01）. [Conclusion] RT-PCR detection of the expression of CEAmRN and DDCmRNA in peritoneal lavage fluid in patients with gastrie cancer can be used to predict the peritoneal micrometastases in patients with gastric caneer.

关 键 词：胃癌 逆转录聚合酶链式反应 CEAMRNA HpamRNA MMP-7mRNA 

分 类 号：R753.2[医药卫生—皮肤病学与性病学]