广西西北部高原地区柿种质资源调查及遗传多样性分析  

Investigation and Analysis on the Genetic Diversity of Persimmon Germplasms in Plateau of Northwest Guangxi

作　　者：邓立宝[1,2] 何新华[1,3] 李天文[1] 胡颖[1] 

机构地区：[1]广西大学农学院,南宁530004 [2]广西百色国家农业科技园区管委会,广西百色533612 [3]广西作物遗传改良生物技术重点开放实验室,南宁530007

出　　处：《园艺学报》2012年第2期215-224,共10页Acta Horticulturae Sinica

摘　　要：调查和研究了广西西北部云贵高原地区柿种质资源状况,并对收集到的柿种质进行表型系统聚类和SCoT遗传多样性分析。结果表明,广西西北部云贵高原地区柿种质资源较丰富,至少拥有柿、油柿和野柿3个种31个不同品种和类型,部分地方仍保存有原始的野生种。不同柿种质在形态和DNA水平上都表现出很大的变异,具有丰富的多样性。35个相对独立的表型性状被用于柿种质的Q型系统聚类分析,在欧氏距离D=9.8处,31份种质被分为5组,与果形及种质类型在一定程度上相吻合。13条SCoT多态性引物可从31份柿种质中扩增出161个DNA位点,多态性带比例为95.65%;UPGMA聚类分析显示,SCoT标记能将31份柿种质完全区分开,在相似系数0.686的水平将其分为5大类,与表型分析结果基本一致。The germplasm resources of persimmon in Yungui plateau of northwest Guangxi were investigated and researched.The collected germplams which included original wild type were analyzed by morphology and SCoT molecular marker.The results showed that Yungui plateau of northwest Guangxi possessed rich persimmon germplasm resources,31 different accessions of germplasm which belonged to three species Diospyros kaki Thunb.,D.oleifera Cheng and D.kaki var.silverstris Mak.were corrected.The corrected persimmon germplasms had large variations on morphology as well as at the level of DNA.Thirty-five independence morphological characters were used to conduct the Q-style systematic clustering analysis and the 31 accessions of persimmon germplasm were divided into 5 groups at the Euclidean distance of 9.8,which accorded with their fruit shapes and germplasm types.Thirteen primers were used to amplify the persimmon genome DNAs and 161 SCoT loci were produced,154 of which were polymorphic loci and with a polymorphism rate of 95.65%.The cluster analysis conducted by UPGMA showed that 31 accessions of persimmon germplasm could be distinguished from each other and were divided into five groups at the similarity coefficient of 0.686,which was similar to the result of morphology analysis.

关 键 词：柿 广西 种质资源 SCoT 遗传多样性 

分 类 号：S665.2[农业科学—果树学]