短期夜间低温对栽培番茄和野生番茄果实蔗糖代谢的影响  

Responses to Short-term Low Night Temperature of Sucrose-metabolizing in Domesticated Tomato and Wild Relative

作　　者：齐红岩[1] 姜岩岩[1] 华利静[1] 

机构地区：[1]沈阳农业大学园艺学院,设施园艺省部共建教育部重点实验室,辽宁省设施园艺重点实验室,沈阳110866

出　　处：《园艺学报》2012年第2期281-288,共8页Acta Horticulturae Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2009CB119000);国家‘十二五’科技支撑计划项目(2011BAD12B03);辽宁省重大科技攻关项目(2010215003)

摘　　要：在人工气候室内以低温敏感的栽培番茄(Solanum lycopersicon)‘Money Maker’和耐低温的野生番茄(S.habrochaites)‘LA1777’为试材,研究了短期(4d)夜间15℃处理和9℃、6℃及其恢复处理对番茄果实糖含量和蔗糖代谢相关酶活性的变化。结果表明:6℃处理0.5d,耐低温的番茄(‘LA1777’)果实中果糖和葡萄糖含量升高,低温敏感番茄(‘Money Maker’)果实中两种糖含量无显著变化。6℃处理‘Money Maker’中蔗糖和淀粉的含量显著降低,而‘LA1777’中含量提高。在恢复期,两种番茄的蔗糖含量均恢复到对照水平,6℃处理的葡萄糖和果糖未恢复到对照水平,而9℃与对照无显著差异。6℃处理降低两种番茄果实中转化酶和蔗糖合成酶(SS分解方向)的活性,无法恢复至对照水平,‘Money Maker’果实中SS合成方向的活性和蔗糖磷酸合成酶(SPS)活性提高,而‘LA1777’变化不显著。说明短期夜间低温条件下耐低温的番茄是通过提高果实中总的碳水化合物含量来适应低温胁迫的,而低温敏感的番茄则是降低果实中碳水化合物含量来响应低温。Chilling-sensitive tomato（Solanum lycopersicon）＇Money Maker＇and chilling-tolerant wild tomato（S.habrochaites）＇LA1777＇were cultivated in phytotron,and effects of low night temperature of 15℃（control）,9℃and 6℃and recovery on carbohydrate content and sucrose-metabolizing enzymes activities in tomato fruits were investigated.The results showed that fructose and glucose levels increased in chilling-tolerant wild tomato（＇LA1777＇）fruits after 0.5 d treatment at 6℃,while with no significant change in chilling-sensitive tomato（＇Money Maker＇）fruits.The sucrose and starch levels in＇Money Maker＇fruits decreased significantly at 6℃,but increased in＇LA1777＇.During the recovery period,the sucrose levels in two types tomato fruits both recovered to control level.The fructose and glucose contents in fruits after low night temperature-treatment could not recovered to the control level,except for 9℃.The invertase and sucrose synthase（SS hydrolysis direction）activities decreased in two types of tomatoes at 6℃,and could not recovery to the control level;However,the sucrose synthase synthesis direction and sucrose phosphate synthase（SPS）activities increased in chilling-sensitive tomato（＇Money Maker＇）fruits while with no significant change in chilling-tolerant wild tomat（o＇LA1777＇）.These results demonstrate that the chilling-tolerant tomato（＇LA1777＇）enhances higher level of total carbohydrate content to acclimate chilling stress under short-time low night temperature,while chilling-sensitive tomato responses to low temperature by decreasing it.

关 键 词：番茄 夜间低温 糖 蔗糖代谢相关酶 

分 类 号：S641.2[农业科学—蔬菜学]