慢性鼻-鼻窦炎纤毛上皮细菌生物膜超微形态学观察  

Micromorphological observation of bacterial biofilms on ciliated epithelia of chronic rhinosinusitis

作　　者：杨海斌[1] 郭永清[1] 余明强[1] 

机构地区：[1]厦门大学附属中山医院耳鼻咽喉科,福建厦门361004

出　　处：《临床耳鼻咽喉头颈外科杂志》2012年第5期194-196,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:观察慢性鼻-鼻窦炎(CRS)纤毛上皮细菌生物膜的超微形态学特点。方法:取4例行鼻内镜鼻窦手术的CRS患者中鼻甲黏膜,每个标本约4mm×4mm大小。经4%多聚甲醛固定24h、1%四氧化锇固定2h、乙醇梯度脱水、二氧化碳干燥、表面喷金镀膜后,用扫描电子显微镜观察标本超微形态。结果:4例标本均发现细菌生物膜的存在,生物膜主要形成于纤毛表面,细菌的鞭毛与纤毛缠绕;可见由多种细菌或细菌和真菌构成的混合感染。结论:细菌生物膜可以形成于CRS纤毛上皮上。Objective：To observe the micromorphological characteristic of bacterial biofilm on mucosa of chronic rhinosinusitis（CRS）.Method：Mucosa samples of middle turbinate were obtained from 4 patients of CRS during ESS.The size of each sample was about 4 mm×4 mm.The samples were fixed in 4% paraformaldehyde for 24 hours,then fixed in 1% osmium tetroxide for 2 hours,graded dehydration with ethanol,dried with carbon dioxide and sputter coated with gold.The ultrastructure of these samples was observed by scanning electron microscope.Result：Bacterial biofilms were found on samples in all 4 patients.The biofilms were mainly formed on the surface of cilia.The bacterial flagella and cilia were intertwined.The biofilms could be found in a lot kinds of bacterial infections or mixed infections which were caused by multiple bacteria and fungi.Conclusion：Bacterial biofilm could be formed on ciliated epithelia.

关 键 词：鼻窦炎 细菌生物膜 纤毛 

分 类 号：R765.4[医药卫生—耳鼻咽喉科]