血管内皮生长因子165重组质粒pcDNA/V促兔椎体终板血管生成的实验观察  

Effects of vascularendothelial growth factor vector on vascular buds of vertebral cartilaginous endplate in rabbits

作　　者：徐宏光[1] 丁国正[1] 陈小虎[2] 王弘[1] 王凌挺[1] 陈学武[1] 

机构地区：[1]皖南医学院附属弋矶山医院脊柱外科,安徽芜湖241001 [2]安徽省淮南市第一人民医院骨科

出　　处：《中华医学杂志》2012年第7期491-495,共5页National Medical Journal of China

基　　金：国家自然科学基金（30973025）;安徽省教育厅自然科学基金重点项目（KJ2010A320）

摘　　要：目的 将构建的pcDNA3.1-VEGF165质粒直接注射于退变的椎间盘以探讨其对软骨终板内微血管和椎间盘的影响.方法 选择6～8个月龄清洁级新西兰兔共20只,分为pcDNA3.1（＋）空质粒组,10只兔（对照组）；pcDNA3.1-VEGF165组,10只兔（实验组）.注射麻醉后,沿左侧12肋末向下至髂嵴做纵切口,推开腰椎间盘前方筋膜和前纵韧带,以椎体前方的纵性骨棘为标志从腰椎间盘前方中心点处垂直进针,分别以微量注射器注入20μl pcDNA3.1（＋）空质粒和pcDNA3.1-VEGF165,于术后4、8周摄片、取材,行X线、组织学及血管铸型后扫描电镜观察.结果 X线摄片观察,术后8周实验组较对照组终板钙化较轻；组织学观察,术后8周实验组退变较对照组明显减轻；扫描电镜观察,VEGF165注射后4周和8周时,明显增加了终板软骨内微血管的数量和直径,并且内层纤维环区较髓核区增加更为明显,随着时间的延长,微血管的数量和直径虽然会随之减小,但仍然明显多于对照组.结论 pcDNA3.1-VEGF165质粒椎间盘内直接注射,可经软骨终板途径,增加终板内微血管的数量和直径,有效的延缓软骨终板的钙化和椎间盘的退变.Objective To directly inject recombinant pcDNA3.1-VEGF165 plasmid into degeneration interverbral disc and explore its effects on vascular buds of vertebral cartilage endplate and intervertebral disc in rabbits.Methods Rabbits were randomly assigned into the experimental and control groups （n =10 each）.For the experimental group,the animals were anesthetized and the front vertebral body exposed.With the longitudinal ossature of front vertebral body of lumbar vertebrae as a mark,a needle was inserted at the central point of the front fourth and fifth lumbar intervertebral disc and 20 μl pcDNA3.1-VEGF165 injected.For the control group,20 μl pcDNA3.1 was injected.At Weeks 4 and 8 post-injection,the changes of vertebral cartilage endplate were monitored by radiograph,histology and scanning electron microscopy.Results The vertebral cartilage endplate calcification and degeneration in the experimental group were less pronounced than that in the control group at Week 8 post-operation.The average number and diameter of vascular buds obviously increased in the experimental group at Weeks 4 and 8 post-operation.The number and diameter of vascular buds in the region of inner annulus increased compared with those in the area near nucleus pulposus.Conclusion The pcDNA3.1-VEGF165 plasmid may promote the vascular buds of vertebral cartilage endplate by increasing their average number and diameter and arresting the intervertebral disc degeneration.

关 键 词：椎间盘 质粒 内皮 血管 内皮生长因子 软骨 

分 类 号：R681.53[医药卫生—骨科学]