小鼠早期完整胚胎诱导子宫内膜白血病抑制因子和整合素β_3表达并提高子宫容受性  

Mouse early integral embryo induces expression of endometrial integrin β_3 and leukaemia-inhibitory factor,and improves uterine receptivity in mice

作　　者：李婷婷[1] 方丛[1] 贾磊[1] 岳超敏[1] 

机构地区：[1]中山大学附属第六医院生殖医学研究中心,广东广州510655

出　　处：《中国病理生理杂志》2012年第2期193-200,共8页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81070495);广东省自然科学基金资助项目(No.9151018201000018)

摘　　要：目的:探讨小鼠早期胚胎诱导子宫内膜容受性改变的空间结构,确定胚胎各个部分能否引起子宫内膜容受性中白血病抑制因子(LIF)和整合素β3改变。方法:选用6～8周昆明雌鼠,体外实验分为子宫内膜培养前组、子宫内膜单纯培养组、子宫内膜全胚胎共培养组、子宫内膜卵裂球共培养组、子宫内膜透明带共培养组,各组培养2 d后收集子宫内膜行下一步检测;体内实验分为胚胎移植前组、单纯培养液移植组、全胚胎移植组、卵裂球移植组、透明带移植组及正常妊娠未干预组,移植后2 d收集子宫内膜行下一步检测。荧光定量PCR检测整合素β3和LIF mRNA表达;免疫组织化学及Western blotting检测整合素β3和LIF的蛋白表达部位及表达水平。结果:体外实验子宫内膜全胚胎共培养组中子宫内膜的整合素β3和LIF表达显著高于其它组,体内实验正常妊娠未干预组的整合素β3和LIF表达显著高于其它组。结论:完整胚胎可显著提升小鼠孕早期子宫内膜容受性因子整合素β3和LIF的表达,而单纯透明带或卵裂球则不能明显增加整合素β3和LIF的表达。早期胚胎诱导子宫内膜容受性可能需要完整胚胎结构的存在。AIM： To investigate the changes of endometrial receptivity under the effect of mouse embryo both in vitro and in vivo,and to figure out which part of the embryo induces the change. METHODS： Scanning electron microscope was applied to observe the pinpode formation on day 4 endometrium both in vitro and in vivo.The expression of integrin β3 and leukaemia-inhibitory factor（LIF） on day 2 pregnant mouse endometrium,day 4 endometrium after co-culture for 2 d with day 2 embryo,blastomere and zona pellucida as well as control group in vitro were detected by the methods of fluorescent quantitative PCR,immunohistochemistry and Western blotting.The same tests were conducted in the in vivo part of the experiment with the integral embryo or different parts of the embryo being transferred to pseudopregnant mouse uterus.On day 4 of the pregnancy,the endometrium was extracted to carry out the tests. RESULTS： After co-cultured for 2 d with whole embryo,the experssion of integrin β3 and LIF was higher than that in any other group in the in vitro part.The expression of integrin β3 and LIF on day 4 of normal pregnancy was higher than that in any other group in the in vivo experiments. CONCLUSION： Mouse embryo as a whole is able to induce better endometrial receptivity,while any separated part of embryo,such as blastomere or zona pellucida,couldn＇t.

关 键 词：胚胎 整合素Β3 白血病抑制因子 子宫内膜容受性 

分 类 号：R321.2[医药卫生—人体解剖和组织胚胎学]