三氯乙烯药疹样皮炎病人外周血c-fos、c-myc、K-ras和p53 mRNA表达水平的研究  

mRNA expression of oncogenes in patients with allergic dermatitis induced by trichloroethylene

作　　者：徐新云[1] 刘月峰[1] 易娟 周丽[1] 黄新凤[1] 毛吉炎[1] 毛侃琅[1] 

机构地区：[1]深圳市疾病预防控制中心,深圳518055 [2]深圳市职业病防治院,深圳518001

出　　处：《癌变．畸变．突变》2012年第1期56-59,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：深圳市科技计划项目(201101015)

摘　　要：目的:探讨三氯乙烯(trichloroethylene,TCE)药疹样皮炎病人外周血癌基因c-fos、c-myc、K-ras、p53 mRNA表达水平的变化情况。方法:分别抽取4例健康人(对照组)及4例三氯乙烯致变态反应病人(病例组)抗凝外周全血,采用实时荧光定量PCR(real-time quantitative PCR)技术检测外周血中c-fos、c-myc、K-ras和p53 mRNA的表达水平。结果:与健康对照者比较,三氯乙烯药疹样皮炎病人外周血c-fos mRNA表达水平升高352%,c-myc mRNA升高41%,K-ras mRNA升高136%,p53 mRNA升高64%,两组间上述基因mRNA表达水平的差异均有统计学意义(P<0.01或P<0.05)。结论:三氯乙烯药疹样皮炎病人外周血癌基因表达水平增加,提示三氯乙烯可能有一定的致癌风险。OBJECTIVE：To study mRNA expression of oncogenes（c-fos,c-myc,K-ras,p53） in peripheral blood of patients allergic to trichloroethylene（TCE）.METHODS：Peripheral blood samples were collected from healthy workers（control group） and allergic patients（case group）.Real-time quantitative PCR was applied to detect mRNA expression of oncogenes c-fos,c-myc,K-ras,p53.RESULTS：The level of c-fos mRNA expression increased by 352%in TCE patients when compared with control（P0.01）,c-myc increased by 41%,K-ras by 136%and p53 increased by 64%.mRNA expression levels of these oncogenes showed significant differences between case and control groups（P0.01 or P0.05）.CONCLUSION：Trichlorcethylene could induce oncogene expression in patients with allergic dermatitis,indicating that TCE might be potentially carcinogenic.

关 键 词：三氯乙烯 外周血 原癌基因 荧光定量PCR 致癌性 

分 类 号：R135.7[医药卫生—劳动卫生]