大鼠腭中缝牵张成骨中骨形态发生蛋白7的作用  

Role of bone morphogenetic protein-7 in the distraction osteogenesis of rat mid-palatal suture

作　　者：李威[1] 李鹍[2] 王培军[1] 韩晶莹[1] 

机构地区：[1]哈尔滨医科大学第二临床医学院口腔正畸科,黑龙江省哈尔滨市150086 [2]哈尔滨医科大学第二临床医学院科研科,黑龙江省哈尔滨市150086

出　　处：《中国组织工程研究与临床康复》2012年第2期211-214,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：黑龙江省自然科学基金(D20965);市科委青年科技创新人才基金资助项目(2010RFQXS113)~~

摘　　要：背景:已有实验证实,骨形态发生蛋白7可诱导骨组织和软骨组织的形成,在牵张成骨的过程中促进骨组织形成。目的:测定骨形态发生蛋白7在腭中缝牵张成骨中的作用及机械扩张力作用下的变化。方法:采用自制双眼扩弓簧对大鼠施加扩张力,建立上颌骨扩张大鼠模型。分别于建模后第1,3,5,7,9,12,14天随机选取大鼠取腭中缝组织,实时定量PCR法测定目的基因的变化规律。结果与结论:建模后第3,9,12,14天大鼠腭中缝组织骨形态发生蛋白7mRNA的表达逐渐升高(P<0.05)。结果证实,大鼠腭中缝牵张成骨时,骨形态发生蛋白7mRNA在机械力刺激下表达明显上调。BACKGROUND： Experiments have confirmed that bone morphogenetic protein-7 can induce bone and cartilage formation; it can also promote bone formation in the process of distraction osteogenesis. OBJECTIVE： To explore the role of bone morphogenetic protein-7 in the suture distraction osteogenesis of rat mid-palatal suture and to detect the changes of bone morphogenetic protein-7 under the mechanical expansion force. METHODS： Expansionary force was applied on rats using a self-made expansing arch spring for binoculus to construct maxillary expansion rat model. Rat mid-palatal sutures were randomly collected on 1, 3, 5, 7, 9, 12 and 14 days after model construction; real-time PCR was performed to detect the changing regularities of the target genes. RESULTS AND CONCLUSION： The expression of the bone morphogenetic protein-7 mRNA in the rat mid-palatal sutures increased gradually on 3, 9, 12 and 14 days after model construction （P 0.05）. These findings demonstrate that bone morphogenetic protein-7 mRNA expression is significantly increased in the distraction osteogenesis process of rat mid-palatal sutures under the stimulus of mechanical force.

关 键 词：骨形态发生蛋白7 牵张成骨 大鼠 腭中缝 骨组织构建 

分 类 号：R318[医药卫生—生物医学工程]