醒脑静抑制重组人肿瘤坏死因子介导的人脐静脉内皮细胞增殖  

Xingnaojing inhibits the proliferation of human umbilical cord vein endothelial cells mediated by recombinant human tumor necrosis factor

作　　者：欧阳海春 吴沃栋[2] 钟冬梅[3] 吴焱贤 李文杰[2] 陈晞明[2] 

机构地区：[1]佛山市顺德区第一人民医院心血管内科,广东省佛山市528300 [2]广州医学院第三附属医院心血管内科,广东省广州市510150 [3]广州医学院第三附属医院中医科,广东省广州市510150

出　　处：《中国组织工程研究与临床康复》2012年第2期239-242,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广州市中医药局中西医结合科研课题(2004A014):"安宫牛黄丸及类方干预急性心梗炎性因子及纤溶的实验与临床研究";广东省中医药局建设中医药强省科研课题(2060063):"安宫牛黄丸及类方干预急性心梗炎性因子及纤溶的实验与临床研究"~~

摘　　要：背景：前期研究将传统名方＂安宫牛黄丸＂经提取精制而成的新型水溶性静脉注射液醒脑静注射液应用于临床治疗冠心病并取得很好的疗效,但其具体机制未完全明确。目的：观察醒脑静对重组人肿瘤坏死因子介导的人脐静脉内皮细胞增殖的影响,探讨醒脑静抑制血管内皮细胞损伤增生的应用价值。方法：取3~5代人脐静脉内皮细胞,在培养基中添加10μg/L重组人肿瘤坏死因子α诱导增殖,醒脑静组加入不同浓度的醒脑静干预,阳性对照组添加氟伐他汀,并设立单纯细胞培养的空白对照组。结果与结论：倒置相差显微镜下可见不同浓度的醒脑静均可使人脐静脉内皮细胞呈现胞浆收缩,贴壁细胞减少,坏死细胞增多。醒脑静组的细胞增殖率低于肿瘤坏死因子α组（P〈0.05）,且呈剂量及作用时间依赖关系。证实醒脑静能有效抑制重组人肿瘤坏死因子α介导的人脐静脉内皮细胞增殖。BACKGROUND： Xingnaojing （XNJ） water solubility intravenous injection is one of prescription similar with Angong Niuhuang pill which has been a most great reputation Chinese traditional medicine. Xingnaojing is used in clinical treatment for coronary heart disease patients and has achieved good results. However, the mechanism has not been cleared. OBJECTIVE： To investigate the inhibition effects of XNJ on the proliferation of human umbilical vein endothelial cells （HUVECs） mediated by recombinant human tumor necrosis factor α （rhTNF-α）. To evaluate the value of XNJ on the proliferation of vascular endothelial cells impaired. METHODS： The passage 3-5 vein endothelial cells were obtained, and were induced to proliferate by adding 10 μg/L rhTNF-α in the culture medium. XNJ in different concentration were injected in the XNJ group, fluvastatin as positive control group, and blank control group had been respectively incubated with HUVECs mediated by rhTNF-α. RESULTS AND CONCLUSION： The endochylema was shrinked, adherent cells were decreased and necrosis cells were increased in HUVECs of XNJ group under inverted phase microscope. The growth rate of HUNVCs proliferation was significantly lower in XNJ group in each different concentration than that in control group （P 0.05）, and showed dose and time dependent manner. XNJ can effectively inhibit the proliferation of HUVECs mediated by rhTNF-α.

关 键 词：醒脑静 内皮细胞 肿瘤坏死因子 增殖 安宫牛黄丸 

分 类 号：R318[医药卫生—生物医学工程]