血管内皮生长因子与促血管生成素1对大鼠血管内皮细胞的作用  

Effect of vascular endothelial growth factor and angiopoietin-1 on rat vascular endothelial cells

作　　者：卢秀珍[1,2] 毕宏生[1,2] 崔彦[1,2] 

机构地区：[1]山东中医药大学第二附属医院 [2]山东中医药大学眼科中心,山东省济南市250002

出　　处：《中国组织工程研究与临床康复》2012年第2期247-251,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：山东省自然科学基金资助项目(Y2006C101)~~

摘　　要：背景:血管内皮生长因子、促血管生成素1是血管形成过程中始动并且使之持续的重要因子,研究其对血管内皮细胞的作用具有重要的意义。目的:观察血管内皮生长因子与促血管生成素1对培养血管内皮细胞迁移与增殖能力的影响,并探讨其在血管生成方面的作用机制。方法:在大鼠脐静脉内皮细胞内单独或联合加入血管内皮生长因子、促血管生成素1后,划痕实验和MTT检测对细胞迁移与增殖的影响,观察内皮细胞形态、活性、迁移能力。结果与结论:划痕实验显示单独血管内皮生长因子作用时,与空白对照组细胞迁移无明显差异,单独促血管生成素1作用时,不仅不能增加细胞的迁移作用,反较空白对照组有所减弱,当血管内皮生长因子与促血管生成素1联合作用时,细胞迁移较空白对照组明显增强;MTT实验结果表明:单纯加入血管内皮生长因子或促血管生成素1,均不能起到有效促进内皮细胞增殖的作用;联合应用血管内皮生长因子及促血管生成素1可有效促进增殖。结果可见当血管内皮生长因子与促血管生成素1联合应用时,才能有效促内皮细胞迁移与增殖,发挥促血管生成作用。BACKGROUND： Vascular endothelial growth factor and angiopoietin-1 play an important role in the initiating and ongoing process of angiogenesis. Study on the effects of vascular endothelial growth factor and angiopoietin-1 on vascular endothelial cells is significant. OBJECTIVE： To explore the effect of vascular endothelial growth factor and angiopoietin-1 on the transfer and proliferation ability in rat vascular endothelial cells and to explore its mechanism on angiogenesis. METHODS： Rat umbilical vein endothelial cells were treated with vascular endothelial growth factor and angiopoietin-1 respectively or in combination. Scratch test and MTT assay were used to detect the cell transfer and proliferation ability. The morphology, activity and transfer ability of endothelial cells were observed. RESULTS AND CONCLUSION： According to scratch test, there was no significant difference in cell transfer ability between the cells treated with vascular endothelial growth factor alone and the control cells. The cell transfer ability after treated with angiopoietin-1 individually did not increase; on the contrary, it was weakened compared with that of the control group. The transfer ability in cells treated with vascular endothelial growth factor combined with angiopoietin-1 improved significantly compared with that of the control group. According to MTT assay, neither vascular endothelial growth factor nor angiopoietin-1 was found to improve the proliferation ability in rat umbilical vein endothelial cells. Combination of vascular endothelial growth factor and angiopoietin-1 significantly improved the proliferation of rat umbilical vein endothelial cells. These findings indicate that the transfer ability, proliferation ability and angiogenesis in rat umbilical vein endothelial cells are only improved by the combination of vascular endothelial growth factor and angiopoietin-1.

关 键 词：血管内皮生长因子 促血管生成素1 大鼠脐静脉内皮细胞 血管形成 脉络膜新生血管 

分 类 号：R318[医药卫生—生物医学工程]