绵羊β3肾上腺素能受体基因在脂肪组织中的表达研究  

Expression of β3-Adrenergic Receptor Gene in Ovine Adipose Tissues

作　　者：武建亮[1,2] 乔利英[1] 刘建华[1] 高中元[1] 林婄婄[1] 刘文忠[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]北京养猪育种中心,北京100194

出　　处：《畜牧兽医学报》2012年第2期196-203,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30972084);国家"十一五"科技支撑计划课题(2008BADB2B04);山西省科技攻关项目(011029)

摘　　要：旨在对绵羊β3肾上腺素能受体基因在脂肪组织中的表达进行研究。本研究通过real-time PCR和免疫组化的方法检测了2个绵羊群体皮下脂肪、大网膜、小网膜、腹膜后脂肪、肠系膜和肾周等6种脂肪组织中ADRB3基因mRNA及其蛋白的表达量与分布情况。结果表明:ADRB3蛋白位于脂肪细胞的细胞膜中。ADRB3基因mRNA及其蛋白在皮下脂肪组织的表达丰度最小(0.159和0.139),在腹膜后脂肪组织的表达丰度最大(2.911和2.225),深层脂肪组织中ADRB3基因mRNA表达量要显著高于皮下脂肪组织(P<0.05),表明皮下脂肪组织的脂肪分解率要低于深层脂肪组织。品种对ADRB3基因mRNA的表达没有显著影响,但对于ADRB3蛋白的表达影响显著。不同脂肪组织中ADRB3表达丰度的差异反映了山西肉用绵羊的遗传稳定性较差。本研究的结果与已知的ADRB3调节脂肪分解和产热的功能是一致的,为利用ADRB3基因作为候选基因进行绵羊新品种的培育提供理论依据。Abstract： The aim of this study was to study the expression of β3-andrenergic receptor gene in ovine adipose tissues. In this study, South African Mutton Merino （SAMM） and Shanxi Dam Line （SXDL） ovines were used to study the distribution and expression of ADRB3 in subcutaneous, omental, small omental, retroperitoneal, mesenteric and perirenal fat tissues of sheep by immu- nohistochemical technique and real-time polymerase chain reaction. The result showed that AD- BR3 was located in the membrane of adipose cell. ADRB3 mRNA and protein were detected in all studied tissues, the lowest expression of ADRB3 mRNA and protein were detected in subcutane- ous fat （0. 159 and 0. 139, respectively）, and the highest expression in retroperitoneal fat （2. 911 and 2. 225, respectively）, and higher expression level were found in great omental and retroper- itoneal fat than that in subcutaneous fat （P〈0.05）. Significant difference （P〈0.05） in expres- sion level of ADBR3 protein was detected between the two sheep populations. The variation in expression of ovine ADRB3 in different adipose tissues reflected the difference between breeds, which suggested there was less genetic stability in Shanxi Dam Line. These findings are consist- ent with the known function of ADRB3 in mediating lipolysis and homeostasis in adipose tissues.It may provide theoretical foundation for breeding of new sheep breed using ADRB3 gene as a candidate gene.

关 键 词：ADRB3基因 绵羊 MRNA表达 免疫组化 脂肪组织 

分 类 号：S826[农业科学—畜牧学] Q786[农业科学—畜牧兽医]