中国美利奴羊不同MBL型个体感染绵羊肺炎支原体的免疫应答分析  

Immunological Response Analysis of Chinese Merino Sheep with Different MBL Genotype Infected with Mycoplasma pneumonia

作　　者：张慧[1] 赵宗胜[1] 赵凤[1] 余鹏[1] 班谦[1] 王遵宝[1] 

机构地区：[1]新疆石河子大学动物科技学院,石河子832000

出　　处：《畜牧兽医学报》2012年第2期306-313,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目(30660125)

摘　　要：为探讨中国美利奴羊不同甘露(聚)糖结合凝集素(MBL)型个体感染绵羊肺炎支原体的免疫应答变化,对4种不同MBL型个体进行MBL水平测定,选择其中20只作为对照组,50只为试验组,在相同饲养条件下试验组人工感染绵羊肺炎支原体,分别在攻毒前1d(-1d)、攻毒后1d(1d)、1周(7d)、2周(14d)、3周(21d)用ELISA方法定量分析血清中TNF-α、IFN-γ、补体C1、C3水平。结果显示,A型和B型其MBL浓度较低,C型MBL浓度较高,与对照组相比,在攻毒后1周,A型和B型IFN-γ表达显著降低(P<0.05),攻毒后2周,补体C3表达显著降低,TNF-α升高显著(P<0.05);与A型和B型相比,在攻毒后1周,C型IFN-γ表达增加(P<0.05),在攻毒后2周,补体C3表达增加、TNF-α显著降低(P<0.05),补体C1各组均不显著。结论:低血清MBL浓度与绵羊支原体肺炎有一定的相关性,不同基因型之间其TNF-α、IFN-γ、补体C1、C3水平有差异,低浓度MBL绵羊更易发生比较严重的炎症反应。The aim of this study was to analyze the immunological response to Mycoplasma pneu- monia in Chinese Merino Sheep with different MBL genotype. MBL levels were measured in the four different individuals, 50 healthy Chinese Merino sheep with different genotypes were artifi- cally infected with M. pneumonia under the same raising condition, while 20 sheep were the con- trol group, the content of TNF-a, IFN-Υ, C1, C3 in the serum were quantitatively assayed by EL1SA on 1 day before infection （-1 d）, and on 1 （the day of infection）, 7, 14 and 21 day. The results showed that. 1）A and B type had low concentration of MBL, while C type＇s MBL level was higher 2） One week after infection, the percentage of IFN-Υ in the MBL A and MBL B was significantly lower than that of control group（P〈0.05）, two weeks later, the level of C3 was lower and the TNF-a was significantly higher（P〈0.05）. Compared with the MBL A and MBL B at one week after infection, the level of IFN-7 was significantly higher in the group of high con- centration of MBL, two weeks later, the level of C3 increased and the level of TNF-Υwas signifi- cantly lower （P〈0. 05）, the percentage of C1 had no significant difference among the groups （P〉0.05）. Conclusion： there maybe some correlation between the lower concentration of MBL plas- ma and mycoplasma pneumonia, while there were some difference among the level of TNF-α,IFN-Υ）＇, C1, C3, the low MBL ones were likely to have more severe inflammatory response.

关 键 词：中国美利奴羊 绵羊支原体肺炎 MBL 细胞因子 

分 类 号：S852.62[农业科学—基础兽医学]