凋亡相关蛋白在小鼠海马发育和老化中的表达  

Expression of apoptosis related protein in the development and aging of mice hippocampus

作　　者：邵姝元[1] 李潮[2] 张敬坤[3] 张莉[3] 

机构地区：[1]辽宁医学院病理生理学教研室,锦州121001 [2]辽宁医学院附属第一医院,锦州121001 [3]辽宁医学院组织学与胚胎学教研室,锦州121001

出　　处：《解剖学杂志》2012年第1期56-58,62,共4页Chinese Journal of Anatomy

摘　　要：探讨Bcl-2和Bax在C57／BL6小鼠海马发育和老化过程中的表达变化。方法：取胚龄（E）18、20d和生后（P）1、3、5、7、14、21、28d以及2、3、6、15、18个月（M）的CA7／BL6小鼠海马，应用免疫组织化学、免疫印迹法及图像分析技术检测Bcl-2和Bax蛋白的表达。结果：E18d～P7d，海马CA区及齿状回Bcl-2和Bax阳性细胞逐渐密集。P14-P21d，CA区两种阳性细胞稀疏；齿状回Bcl-2阳性细胞逐渐稀疏，Bax阳性细胞P14d密集，P21d稀疏。P28d后均趋于稳定。图像分析显示，E18d-P21d，除12）（3区Bcl-2的平均光密度值无差异外，BcP2和Pax均表现为先增大后降低的趋势。免疫印迹法结果显示，Bcl-2和Bax表达E18d～P7d逐渐增多，P14~P21d减少；P28d后趋于稳定。Bcl-2／Bax在P1d降低，P3d～P5d增高，P7d～P21d降低，P28d后趋于稳定。结论：Bcl-2和Bax在小鼠海马发育早期表达较多，成年及老年期处于较低水平，表明它们可能参与了海马的塑形过程。To observe the changes of expression of Bcl-2 and Bax protein in the development and aging of C57/ BL6 mouse hippocampus. Methods： Embyronic （E） 18, 20 day （d） and postnatal 1, 3, 5, 7, 14, 28 d and 2, 3, 6, 15, 18 month （M） mice were used in this study. Each group included 8 mice. Immunohistochemistry and immunoblot asssay were used to detect the expression of Bcl-2 and Bax protein in the mice hippocampus of different age. Results： From El6 d to P7 d, Bcl-2 and Bax immunoreactive cells increased in the hippocampus. On P14 d-P21 d, the two kinds of immunoreactive cells decreased, except the Bax positive cells in dentate gyrus which increased on P14 d while decreased on P21 d. Then this two kinds of cells remained unchanged after P28 d. Additionally, the average optical density of Bcl-2 and Bax on El8 d-P21 d in- creased firstly and then decreased, except the Bcl-2 in dentate gyrus area. Immunoblotting analysis showed that the expres- sion of Bcl-2 and Bax protein increased on E18 d-P7 d, decreased on P14 d-P21 d, and remained unchanged after P28 d. Bcl- 2/Bax decreased on P1 d, increased on P3 d-P5 d, decreased on P7 d-P21 d, and remained unchanged on P28 d. Conclusion： The expression of Bcl-2 and Bax is high in the early stage of hippocampus development and then low in adult and senectitude. It may be related with the formation of the hippocampus.

关 键 词：发育 海马 BCL-2 BAX 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]